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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Recombinant Trypsin (rTrypsin) OneStep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3158
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    Recombinant Trypsin (rTrypsin)

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Others

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 2.7%；
      Inter-assay: 4.2%

    • Sample type

      Cell culture supernatant; Serum; Heparin Plasma, Citrate Plasma, Cell culture extracts, Tissue extracts

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      4.350 pg/mL

    • Range

      7.81 pg/mL – 500 pg/mL

    • Recovery

      Cell culture supernatant: 97% (Media is 1640 containing 10% fetal bovine serum)
      Serum: 108%
      Heparin Plasma: 92%
      Citrate Plasma: 86%
      Cell culture extracts: 105%
      Tissue extracts: 96%

    • Assay time

      60 minutes

    • Species reactivity

      Porcine
背景介绍

  • Recombinant Trypsin (rTrypsin) is a genetically engineered serine protease (EC 3.4.21.4) produced via recombinant DNA technology, most commonly in microbial host systems such as Pichia pastoris or E. coli, to yield an enzyme that is identical in amino acid sequence to native porcine or bovine trypsin while ensuring an animal-free production process free from contaminants like chymotrypsin and risks of viral or transmissible spongiform encephalopathy (TSE) agents. As a key biocatalyst, it specifically hydrolyzes peptide bonds at the carboxyl termini of lysine and arginine residues, a specificity determined by its conserved catalytic triad (His57, Asp102, Ser195) and the substrate-binding S1 pocket, with its structural integrity maintained by a calcium-binding loop.

  • ELISA

    • Standard curve
      Example of Recombinant Trypsin standard curve in Assay Diluent DA2.

    • Linearity
      The concentrations of Recombinant Trypsin (rTrypsin) were measured and interpolated from the target standard curves and corrected for sample dilution.
      Sample is undiluted samples are as follows: 0.25 % Trypsin-EDTA (2.5 g/L trypsin, 0.38 g/L EDTA·4Na) was 6.25×104 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 33.52 μg/mL in 0.25 % Trypsin-EDTA.

    • To assess the linearity of the assay, four samples were spiked with high concentrations of Recombinant Trypsin (rTrypsin) in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Specificity
      Test the specificity of different serum.

    • Leading Competitor comparison

    • Protocol Diagram

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