产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
产品介绍 评论(0)
抗原名称
IL-1β免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Mk纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8°C as supplied.
Precision
Intra-assay: 1.5%
Inter-assay: 2.0%Sample type
Cell culture supernatant; Serum; PlasmaAssay type
Sandwich (quantitative)Sensitivity
0.170 ng/mLRange
0.31 ng/mL – 20 ng/mLRecovery
Cell culture supernatant: 116%
Monkey Serum: 93%
EDTA Plasma: 100%
Heparin Plasma: 109%Assay time
60 minutesSpecies reactivity
Mk; Hu
Monkey IL-1β (interleukin-1 beta) is a recombinant cytokine derived from non-human primates such as cynomolgus or rhesus macaques. As a key mediator of innate immunity, it plays a central role in promoting inflammatory responses to infection, tissue damage, and autoimmune stimuli. Due to its high structural and functional homology with human IL-1β, monkey IL-1β serves as a critical tool in translational research. It is widely used in immune cell activation assays, inflammasome studies, and preclinical evaluation of anti-inflammatory biologics. The protein’s strong cross-reactivity with human IL-1 receptors enables accurate modeling of human inflammatory diseases in primate systems, providing essential insights into drug efficacy and safety prior to clinical trials.
ELISA
Standard curve
Example of Monkey IL-1β standard curve in Assay Diluent GB4E5.Linearity
#1 sample is undiluted samples are as follows: Monkey PBMC cells stimulated with 10ug/ml PHA for 5days (100%). #2 sample is undiluted samples are as follows: Monkey PBMC cells culture for 5 days (100%).Specificity
Example of Monkey IL-1β, Human IL-1β and Mouse IL-1β standard curve in Assay Diluent GB4E5.Specificity
Test the specificity of different serum.To assess the linearity of the assay, three samples were spiked with high concentrations of Monkey IL-1β in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Protocol Diagram







评论(0)