产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Monkey IL-1β OneStep ELISA Kit

价格 4,200.00 供应商现货 : 3-5个工作日
货号 S0C3151
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产品介绍 评论(0)

产品规格
  • 抗原名称

    IL-1β
  • 免疫原

    Recombinant Protein
  • 抗体类型

    Recombinant mAb
  • 反应种属 ?

    Mk
  • 纯化方式

    Protein A
  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数
    • Precision

      Intra-assay: 1.5%
      Inter-assay: 2.0%
    • Sample type

      Cell culture supernatant; Serum; Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      0.170 ng/mL
    • Range

      0.31 ng/mL – 20 ng/mL
    • Recovery

      Cell culture supernatant: 116%
      Monkey Serum: 93%
      EDTA Plasma: 100%
      Heparin Plasma: 109%
    • Assay time

      60 minutes
    • Species reactivity

      Mk; Hu
背景介绍
  • Monkey IL-1β (interleukin-1 beta) is a recombinant cytokine derived from non-human primates such as cynomolgus or rhesus macaques. As a key mediator of innate immunity, it plays a central role in promoting inflammatory responses to infection, tissue damage, and autoimmune stimuli. Due to its high structural and functional homology with human IL-1β, monkey IL-1β serves as a critical tool in translational research. It is widely used in immune cell activation assays, inflammasome studies, and preclinical evaluation of anti-inflammatory biologics. The protein’s strong cross-reactivity with human IL-1 receptors enables accurate modeling of human inflammatory diseases in primate systems, providing essential insights into drug efficacy and safety prior to clinical trials.

  • ELISA

    • Standard curve
      Example of Monkey IL-1β standard curve in Assay Diluent GB4E5.

    • Linearity
      #1 sample is undiluted samples are as follows: Monkey PBMC cells stimulated with 10ug/ml PHA for 5days (100%). #2 sample is undiluted samples are as follows: Monkey PBMC cells culture for 5 days (100%).

    • Specificity
      Example of Monkey IL-1β, Human IL-1β and Mouse IL-1β standard curve in Assay Diluent GB4E5.

    • Specificity
      Test the specificity of different serum.

    • To assess the linearity of the assay, three samples were spiked with high concentrations of Monkey IL-1β in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Protocol Diagram

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