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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Mouse C4 OneStep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3143
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    C4

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 3.2%；
      Inter-assay: 5.8%

    • Sample type

      Cell culture supernatant; Serum; Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      21.23 pg/mL

    • Range

      78 pg/mL – 5000 pg/mL

    • Recovery

      Cell culture supernatant: 99%
      Serum: 86%
      Plasma: 103%

    • Assay time

      60 minutes

    • Species reactivity

      Mouse
背景介绍

  • Complement component 4 (C4) is a protein involved in the intricate complement system. It serves a number of critical functions in immunity, tolerance, and autoimmunity with the other numerous components. Furthermore, it is a crucial factor in connecting the recognition pathways of the overall system instigated by antibody-antigen (Ab-Ag) complexes to the other effector proteins of the innate immune response. For example, the severity of a dysfunctional complement system can lead to fatal diseases and infections. Complex variations of it can also lead to schizophrenia.

  • ELISA

    • Standard curve
      Example of Mouse C4 standard curve in Assay Diluent A1.

    • Linearity
      The concentrations of Mouse C4 were measured and interpolated from the target standard curves and corrected for sample dilution.
      S#1 sample is undiluted samples are as follows: BALB/c serum was 2×10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 4.8 μg/mL in BALB/c serum.
      P#2 sample is undiluted samples are as follows: BALB/c Plasma -Heparin was 2×10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 153.12 μg/mL in BALB/c Plasma -Heparin.
      S#3 sample is undiluted samples are as follows: CD-1 serum was 4×10^3fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 7.69 μg/mL in BALB/c serum.
      P#4 sample is undiluted samples are as follows: CD-1 Plasma -Heparin was 2×10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 96.46 μg/mL in BALB/c Plasma -Heparin.

    • Specificity
      Example of Mouse C3、Rat C4 and Mouse C4 standard curve in Assay Diluent A1.
      Antigen concentration: 0-5000 pg/ml.

    • Specificity
      Test the specificity of different serum and plasma.

    • To assess the linearity of the assay, the sample was spiked with high concentrations of Mouse C4 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • To assess the linearity of the assay, two samples were spiked with high concentrations of Mouse C4 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Protocol Diagram

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