热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

所有图片（1/8）

Monkey IL-8 Onestep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3142
规格
数量
收藏 分享
产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IL-8

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Mk

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 6.5%；
      Inter-assay: 6.7%

    • Sample type

      Cell culture supernatant; Serum; Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.444 pg/mL

    • Range

      3.13 pg/mL – 200 pg/mL

    • Recovery

      Cell culture supernatant: 90%
      Serum: 111%
      Plasma: 109%

    • Assay time

      60 minutes

    • Species reactivity

      Mk
背景介绍

  • Monkey Interleukin-8 (IL-8) is a pivotal CXC chemokine in non-human primates, functionally conserved and serving as the primary chemoattractant and activator for neutrophils during innate immune responses. It is rapidly released in response to inflammatory stimuli such as infection or injury, directing neutrophil migration, adhesion, and antimicrobial activity to the affected site. This protein is also involved in angiogenesis, influencing both wound healing and pathological processes. Due to its high biological similarity to human IL-8, it is an indispensable translational biomarker in biomedical research, extensively utilized to assess inflammation, evaluate drug safety, study infectious diseases, and develop vaccines in preclinical primate models.

  • ELISA

    • Standard curve
      Example of Monkey IL-8 standard curve in Assay Diluent E1.

    • Linearity
      The concentrations of Monkey IL-8 were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: Monkey PBMC cells stimulated with 10ug/mL PHA for 5days (0.5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 46.70 ng/mL in stimulated monkey PBMC supernatant.
      #2 sample is undiluted samples are as follows: Monkey PBMC cells culture for 5days (2%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 6.87 ng/mL in unstimulated monkey PBMC supernatant.

    • #3 sample is undiluted samples are as follows: Monkey PBMC cells stimulated with 2 ug/mL poly I:C for 24 hours (20%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 807.09 pg/mL in stimulated monkey PBMC supernatant.
      #4 sample is undiluted samples are as follows: Monkey PBMC cells culture for 24 hours (40%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 306.61 pg/mL in unstimulated monkey PBMC supernatant.

    • #5 sample is undiluted samples are as follows: Female monkey serum was 50fold dilution (2%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 7.13 ng/mL in female monkey serum.
      #6 sample is undiluted samples are as follows: Male monkey serum was 50fold dilution (2%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 6.64 ng/mL in male monkey serum.

    • #7 sample is undiluted samples are as follows: Monkey plasma (EDTA) was 10fold dilution (10%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 799.05 pg/mL in monkey plasma (EDTA).

    • Specificity
      Test the specificity of different serum.

    • Leading Competitor comparison

    • Protocol Diagram

评论(0)