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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Mouse TNF-α (serum&plasma) OneStep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3139
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    TNF-α

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 3.6%；
      Inter-assay:4.9%

    • Sample type

      Serum; Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      4.855 pg/mL

    • Range

      15.63 pg/mL – 1000 pg/mL

    • Recovery

      Serum: 95%
      Plasma: 78%

    • Assay time

      60 minutes

    • Species reactivity

      Ms
背景介绍

  • Tumor necrosis factor α (TNF alpha) is an effective pro-inflammatory cytokine, which can kill and inhibit tumor cells. It is mainly produced by activated macrophages and can also be secreted by other types of cells, such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils and neuronal tissues. The members of TNF alpha family exert their cellular effect through two distinct surface receptors of the TNF receptor family, TNFR1and TNFR2. The pleiotropic biological effects of TNF can be attributed to its ability to simultaneously activate multiple signaling pathways in cells. TNF-induced NF-κB activation promotes the growth, invasion and metastasis of cancer cells.

  • ELISA

    • Standard curve
      Example of Mouse TNF-α standard curve in Assay Diluent NE1A5H4.

    • Linearity
      The concentrations of Mouse TNF-α were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: BALB/c Mouse serum(100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 4.88 pg/mL in BALB/c Mouse serum.
      #2 sample is undiluted samples are as follows: C57BL/6 Mouse serum(100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 57.11 pg/mL in C57BL/6 Mouse serum.
      #3 sample is undiluted samples are as follows: CD-1 Mouse serum(100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 19.84 pg/mL in CD-1 Mouse serum.

    • Leading Competitor comparison

    • Protocol Diagram

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