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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Mouse IL-12 P70 Serum OneStep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3137
规格
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产品介绍 评论(0)

产品规格

  • 抗原名称

    IL-12

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 稀释度

    • 稀释度

      [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}, {"application": "ChIP", "dilution": "", "species": ""}]

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 2.6%；
      Inter-assay:2.9%

    • Sample type

      Serum

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      5.205 pg/mL

    • Range

      7.81 pg/mL – 500 pg/mL

    • Recovery

      Serum: 99%

    • Assay time

      60 minutes

    • Species reactivity

      Ms
背景介绍

  • Mouse IL-12 is a critical pro-inflammatory cytokine that bridges innate and adaptive immunity. It is a heterodimeric protein composed of p35 (IL-12A) and p40 (IL-12B) subunits, forming the bioactive IL-12(p70). Primarily produced by antigen-presenting cells like dendritic cells and macrophages upon pathogenic stimulation, its pivotal role is to drive the differentiation of naïve CD4⁺ T cells into T helper 1 (Th1) cells and to potently induce interferon-gamma (IFN-γ) production from both T cells and natural killer (NK) cells. This establishes a robust cellular immune response essential for defense against intracellular pathogens such as viruses and bacteria. In biomedical research, Mouse IL-12 is a cornerstone for studying Th1-mediated immunity, evaluating vaccine efficacy, investigating antitumor immunotherapy strategies, and modeling autoimmune diseases like experimental autoimmune encephalomyelitis (EAE).

  • ELISA

    • Standard curve
      Example of Mouse IL-12 P70 standard curve in Assay Diluent EA2.

    • Linearity
      The concentrations of Mouse IL-12 were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: CD-1 Mouse serum. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 14.72 pg/mL in CD-1 Mouse serum.
      #2 sample is undiluted samples are as follows: BALB/c Mouse serum. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 12.82 pg/mL in BALB/c Mouse serum.
      #3 sample is undiluted samples are as follows: C57BL/c Mouse serum. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 36.29 pg/mL in C57 Mouse serum.

    • To assess the linearity of the assay, sample was spiked with high concentrations of Mouse IL-12 P70 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Leading Competitor comparison

    • Protocol Diagram

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