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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Guinea pig IgG OneStep ELISA Kit

价格 ￥2,000.00 （ 1-2周）
货号 S0C3131
规格
数量
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产品介绍

产品介绍 引用文献(1) 评论(0)

产品规格

  • 抗原名称

    IgG

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 3.5%；
      Inter-assay: 5.9%

    • Sample type

      Cell culture supernatant; Serum; Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      30.616 pg/mL

    • Range

      78.13 pg/mL – 5000 pg/mL

    • Recovery

      Cell culture supernatant: 86%
      Serum: 101%
      Plasma: 103%

    • Assay time

      60 minutes

    • Species reactivity

      Guinea pig
背景介绍

  • Guinea pig immunoglobulin G (IgG) is the predominant class of antibodies found in the serum of guinea pigs (Cavia porcellus), playing a central role in their adaptive immune response by neutralizing pathogens, opsonizing microbes, and activating the complement system. Structurally similar to typical mammalian IgG, it possesses unique biochemical characteristics such as a higher molecular weight (approx. 180 kDa) due to significant glycosylation and strong binding affinity for Protein A, which is uncommon in many other rodent antibodies. These distinctive properties, coupled with the guinea pig's importance as a model for human diseases (e.g., tuberculosis, allergy, and vaccine research), make its IgG highly valuable in biomedical science. It is extensively utilized as a critical reagent in various immunoassays—including ELISA, Western blotting, and immunohistochemistry—serving both as a primary therapeutic research target and as a specific secondary antibody in cross-species experimental designs.

  • ELISA

    • Standard curve
      Example of Guinea pig IgG standard curve in Assay Diluent K1.

    • Linearity
      The concentrations of Guinea pig IgG were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: Guinea pig serum was 2×10^6fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 4.22 mg/mL in Guinea pig serum.

    • Linearity
      The concentrations of Guinea pig IgG were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: Guinea pig plasma (EDTA) was 2×10^6fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 7.82 mg/mL in Guinea pig plasma (EDTA).

    • Linearity
      The concentrations of Guinea pig IgG were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: Guinea pig plasma (Sodium citrate) was 2×10^6fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 6.43 mg/mL in Guinea pig plasma (Sodium citrate).

    • Protocol Diagram

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