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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Human Apolipoprotein E/APOE Serum & Plasma Onestep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3130
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    Apolipoprotein E/APOE

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 2.8%；
      Inter-assay: 4.1%

    • Sample type

      Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.482 ng/mL

    • Range

      0.625 ng/mL – 40 ng/mL

    • Recovery

      Serum: 101%
      Plasma: 104%

    • Assay time

      60 minutes

    • Species reactivity

      Hu
背景介绍

  • Human APOE (Apolipoprotein E) is a key protein involved in lipid metabolism, primarily produced in the liver and brain. It functions as a carrier for cholesterol and other fats, facilitating their transport through the bloodstream. APOE exists in three common isoforms—APOE2, APOE3, and APOE4—which differ by single amino acids. Among these, APOE4 is the strongest genetic risk factor for late-onset Alzheimer's disease, while APOE2 appears to offer some protective effects. The protein also plays a crucial role in neuronal repair and inflammation regulation within the central nervous system. Its interaction with cellular receptors allows cells to take up lipids for membrane maintenance and energy. Overall, APOE is essential for both cardiovascular and brain health, with its genetic variations significantly influencing disease susceptibility.

  • ELISA

    • Standard curve
      Example of Human Apolipoprotein E/APOE standard curve in Assay Diluent EJ.

    • Linearity
      The concentrations of Human Apolipoprotein E/APOE were measured and interpolated from the target standard curves and corrected for sample dilution.
      Sample is undiluted samples are as follows: human plasma was 1000fold dilution (0.1%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 21.40 μg/mL in stimulated human plasma.
      Sample is undiluted samples are as follows: human serum was 1000fold dilution (0.1%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 22.97 μg/mL in stimulated human serum.

    • To assess the linearity of the assay, sample was spiked with high concentrations of Human Apolipoprotein E/APOE in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Protocol Diagram

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