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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Mouse IgG2c Onestep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3128
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IgG2c

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 3.2%；
      Inter-assay: 7.2%

    • Sample type

      Cell culture supernatant; Serum; Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      3.418 pg/mL

    • Range

      39.06 pg/mL – 2500 pg/mL

    • Recovery

      Cell culture supernatant: 89%
      Serum: 83%
      Plasma: 97%

    • Assay time

      60 minutes

    • Species reactivity

      Ms
背景介绍

  • Mouse IgG2c is the functional equivalent and primary Th1-associated antibody isotype in C57BL/6 and related mouse strains, replacing the conventional IgG2a found in other strains like BALB/c. This expression is due to a natural allelic variation in the Igh-1 locus. Functionally, IgG2c mirrors IgG2a by exhibiting high affinity for activating Fc gamma receptors, making it exceptionally potent at eliciting effector mechanisms such as antibody-dependent cellular cytotoxicity (ADCC), phagocytosis (ADCP), and complement-dependent cytotoxicity (CDC). Consequently, it is a critical mediator of protective immunity against intracellular pathogens and a key indicator of a robust Th1-type immune response in these specific mouse strains.

  • ELISA

    • Standard curve
      Example of Mouse IgG2c standard curve in Assay Diluent #1.

    • Linearity
      The concentrations of mouse IgG2c were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: C57BL/C mouse serum was 200,000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 403.69 ug/mL in C57BL/C mouse serum.
      #2 sample is undiluted samples are as follows: CD-1 mouse serum was 200,000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 77.99 ug/mL in CD-1 mouse serum.

    • Linearity
      The concentrations of mouse IgG2c were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: C57BL/C mouse plasma (Heparin) was 200,000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 388.92 ug/mL in C57BL/C mouse plasma (Heparin).
      #2 sample is undiluted samples are as follows: C57BL/C mouse plasma (EDTA) was 200,000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 187.04 ug/mL in C57BL/C mouse plasma (EDTA).
      #3 sample is undiluted samples are as follows: C57BL/C mouse plasma (Sodium citrate) was 200,000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 105.68 ug/mL in C57BL/C mouse plasma (Sodium citrate).

    • Specificity
      Detection of mouse IgG2c in serum from different mouse strains.

    • Protocol Diagram

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