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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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MuLV Titer p30 (Culture media & Plasma) OneStep ELISA Kit

价格 ￥8,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3125
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    MuLV Titer p30

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 3.3%；
      Inter-assay: 5.0%

    • Sample type

      Cell culture supernatant; Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      1.360 pg/mL

    • Range

      4.69 pg/mL – 300 pg/mL

    • Recovery

      Cell culture supernatant: 119%
      Plasma: 84%

    • Assay time

      60 minutes

    • Species reactivity

      Ms
背景介绍

  • The Murine Leukemia Virus (MuLV) p30 protein, also known as the CA (capsid) protein, is the major structural component of the MuLV viral core. As a critical product of the gag gene, it plays an essential role in viral assembly, forming the conical capsid that encases the viral RNA genome and enzymes. Due to its abundance and conservation, the p30 antigen serves as a definitive biomarker for the presence of the virus. Consequently, quantifying p30 levels via ELISA is a well-established and crucial method for determining the physical titer of MuLV particles. This measurement is vital in both virology research, for standardizing viral stocks, and in the biopharmaceutical industry, where it is used to monitor for retrovirus-like contaminants in biologics, such as vaccines and cell therapy products, ensuring patient safety.

  • ELISA

    • Standard curve
      Example of MuLV Titer p30 standard curve in Assay Diluent HA2.

    • Linearity
      The concentrations of MuLV Titer p30 were measured and interpolated from the target standard curves and corrected for sample dilution.
      The sample is undiluted samples are as follows: Virus supernatant was 4×10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 7.12 ug/mL in Virus supernatant.

    • To assess the linearity of the assay, the sample was spiked with high concentrations of MuLV Titer p30 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • To assess the linearity of the assay, the sample was spiked with high concentrations of MuLV Titer p30 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Leading Competitor comparison

    • Protocol Diagram

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