热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

所有图片（1/4）

Mouse IgG3 Onestep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3121
规格
数量
收藏 分享
产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IgG3

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 2.6%；
      Inter-assay: 3.6%

    • Sample type

      Cell culture supernatant; Serum; Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.198 ng/mL

    • Range

      0.78 ng/mL – 50 ng/mL

    • Recovery

      Cell culture supernatant: 98%
      Serum: 105%
      Plasma: 105%

    • Assay time

      60 minutes

    • Species reactivity

      Ms
背景介绍

  • Mouse IgG3 is a unique immunoglobulin subclass with several distinctive properties. Structurally, it has an extended hinge region that confers flexibility but also makes it more susceptible to proteolytic cleavage. A defining feature of IgG3 is its exceptional ability to activate the classical pathway of the complement system, often considered the most potent among all mouse IgG subclasses. However, it binds with very low affinity to most Fc gamma receptors (FcγRs), making it relatively ineffective at FcγR-mediated effector functions like ADCC. IgG3 responses are often prominent against polysaccharide antigens, such as those found in bacterial capsules. It also has a tendency to self-aggregate and form cryoglobulins, especially in certain immune complexes.

  • ELISA

    • Standard curve
      Example of Mouse IgG3 standard curve in Assay Diluent #1.

    • Linearity
      The concentrations of mouse IgG3 were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: CD-1 mouse serum was 2000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 83.11 ug/mL in CD-1 mouse serum.
      #2 sample is undiluted samples are as follows: BABL/C mouse serum was 2000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 100.29 ug/mL in BABL/C mouse serum.
      #3 sample is undiluted samples are as follows: C57BL/C mouse serum was 2000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 129.83 ug/mL in C57BL/C mouse serum.

    • Linearity
      The concentrations of mouse IgG3 were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: C57BL/C mouse plasma (EDTA) was 2000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 110.36 ug/mL in C57BL/C mouse plasma (EDTA).
      #2 sample is undiluted samples are as follows: C57BL/C mouse plasma (Heparin) was 2000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 134.34 ug/mL in C57BL/C mouse plasma (Heparin).
      #3 sample is undiluted samples are as follows: C57BL/C mouse plasma (Sodium citrate) was 4000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 76.64 ug/mL in C57BL/C mouse plasma (Sodium citrate).

    • Protocol Diagram

评论(0)