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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Mouse IgG2b Onestep ELISA Kit

价格 ￥3,000.00 （ 1-2周）
货号 S0C3120
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IgG2b

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 4.7%；
      Inter-assay: 4.8%

    • Sample type

      Cell culture supernatant; Serum; Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.047 ng/mL

    • Range

      0.47 ng/mL – 30 ng/mL

    • Recovery

      Cell culture supernatant: 82%
      Serum: 107%
      Plasma: 99%

    • Assay time

      60 minutes

    • Species reactivity

      Ms
背景介绍

  • Mouse IgG2b is another strongly inflammatory antibody isotype that shares functional similarities with IgG2a, though it is genetically distinct. It effectively binds to several activating Fc gamma receptors (FcγRs), though often with different affinities compared to IgG2a. IgG2b is highly competent at triggering key effector functions, including ADCC, ADCP, and complement activation. Its ability to fix complement is generally considered very strong, often on par with or even exceeding that of IgG2a in certain contexts. IgG2b thus plays a crucial role in the humoral immune response by efficiently opsonizing pathogens for clearance and directly lysing target cells through complement-mediated mechanisms.

  • ELISA

    • Standard curve
      Example of Mouse IgG2b standard curve in Assay Diluent #1.

    • Linearity
      The concentrations of mouse IgG2b were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: CD-1 mouse serum was 8000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 337.57 ug/mL in CD-1 mouse serum.
      #2 sample is undiluted samples are as follows: BABL/C mouse serum was 8000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 42.26 ug/mL in BABL/C mouse serum.
      #3 sample is undiluted samples are as follows: C57BL/C mouse serum was 8000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 573.82 ug/mL in C57BL/C mouse serum.

    • Linearity
      The concentrations of mouse IgG2b were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: C57BL/C mouse plasma (EDTA) was 8000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 345.11 ug/mL in C57BL/C mouse plasma (EDTA).
      #2 sample is undiluted samples are as follows: C57BL/C mouse plasma (Sodium citrate) was 8000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 244.16 ug/mL in C57BL/C mouse plasma (Sodium citrate).

    • Protocol Diagram

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