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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Rat IL-2 (serum + plasma) OneStep ELISA Kit

价格 ￥4,800.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3115
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IL-2

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Rt

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 3.1%；
      Inter-assay: 4.2%

    • Sample type

      Serum; Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      17.83 pg/mL

    • Range

      93.75 pg/mL – 6000 pg/mL

    • Recovery

      Serum: 109%
      Plasma: 111%

    • Assay time

      60 minutes

    • Species reactivity

      Rt
背景介绍

  • Rat IL-2 (Interleukin-2) is a 15–17 kDa glycoprotein and key T-cell growth factor primarily secreted by activated CD4+ T cells, NK cells, and dendritic cells. It drives T-cell proliferation, Th1 differentiation, and NK cell activity while also supporting regulatory T cell (Treg) function for immune tolerance. Signaling through the IL-2 receptor (IL-2Rαβγ) activates JAK-STAT pathways, promoting cell survival and expansion. Rat IL-2 shares ~70% homology with mouse IL-2 but differs significantly from humanIL-2, requiring species-specific detection methods (e.g., ELISA, flow cytometry). It is crucial in research on autoimmunity (e.g., EAE), transplantation, and cancer immunotherapy, with dysregulation linked to immune disorders. Recombinant IL-2 has therapeutic potential but demands rat-specific reagents for accurate study.

  • ELISA

    • Standard curve
      Example of Rat IL-2 standard curve in Assay Diluent #12S2.

    • Linearity
      Linearity
      The concentrations of Rat IL-2 were measured and interpolated from the target standard curves and corrected for sample dilution.
      The sample is undiluted samples are as follows: SHR Rat serum (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 239.11 pg/mL in SHR Rat serum.

    • S#1 sample is undiluted samples are as follows: Lewis Rat serum (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 191.36 pg/mL in Lewis Rat serum.
      P#1 sample is undiluted samples are as follows: Lewis Rat plasma-Heparin (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 274.08 pg/mL in Lewis Rat plasma-Heparin.

    • Protocol Diagram

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