产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
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抗原名称
FSH免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Hu纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8'C as supplied.
Precision
Intra-assay: 2.2%;
Inter-assay: 3.1%Sample type
Serum; Plasma; Cell culture supernatantAssay type
Sandwich (quantitative)Sensitivity
0.066 mIU/mLRange
0.20 mIU/mL –12.48 mIU/mLRecovery
Serum: 110%
Plasma: 105%
Cell culture supernatant: 115%Assay time
60 minutesSpecies reactivity
Hu
Follicle-stimulating hormone (FSH) is a hormone your pituitary gland makes and releases that plays a role in sexual development and reproduction. It affects the function of ovaries and testicles. It is an important hormone that has a few different functions depending on the type of sexual organs (gonads) you have and your age. In males, FSH and LH work together to trigger their testes to begin producing testosterone. This is the hormone responsible for the physical changes of puberty (such as body hair growth and voice deepening) and the production of sperm. In females, FSH and LH trigger their ovaries to begin producing estrogen. This hormone is responsible for physical changes of puberty, like breast development and menstruation.
ELISA
Standard curve
Example of Human FSH standard curve.Linearity
The concentrations of Human FSH were measured and interpolated from the target standard curves and corrected for sample dilution.
S#1 sample is undiluted samples are as follows: human serum (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 13.71 mIU/mL in human serum.
P#1 sample is undiluted samples are as follows: human plasma-heparin (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 4.62 mIU/mL in human plasma-heparin.
P#2 sample is undiluted samples are as follows: human plasma-EDTA (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 6.34 mIU/mL in human plasma-EDTA.To assess the linearity of the assay, three samples were spiked with high concentrations of Human FSH in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay, three samples were spiked with high concentrations of Human FSH in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Leading Competitor comparison
Protocol Diagram







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