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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Human Transthyretin/Prealbumin OneStep ELISA Kit

价格 ￥3,000.00 （ 1-2周）
货号 S0C3101
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    Prealbumin

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 3.6%；
      Inter-assay: 8.5%

    • Sample type

      Cell culture supernatant, Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      6.655 pg/mL

    • Range

      15.63 pg/mL – 1000 pg/mL

    • Recovery

      Cell culture supernatant: 88%
      Serum: 87%
      Plasma-Heparin: 92%

    • Assay time

      60 minutes

    • Species reactivity

      Hu
背景介绍

  • Human Prealbumin, now more commonly referred to as Transthyretin (TTR), is a transport protein primarily synthesized in the liver and the choroid plexus of the brain. It serves two major physiological functions: transporting thyroxine (T4) and forming a complex with retinol-binding protein to carry vitamin A. Notably, it has a short half-life of approximately 2 days, making it a highly sensitive and early indicator of acute nutritional status and systemic inflammation, often measured clinically to assess malnutrition, particularly in conditions like cirrhosis, inflammation, or critical illness. Additionally, certain genetic mutations in the TTR gene can lead to its misfolding and aggregation, resulting in hereditary amyloidosis, a serious disease where amyloid fibrils accumulate in tissues such as the heart and nerves.

  • ELISA

    • Standard curve
      Example of Human Prealbumin standard curve in Assay Diluent #1.

    • Linearity
      The concentrations of Human Prealbumin were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: Human serum was 2.5×10^5fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 238.65 ug/mL in human serum.
      #2 sample is undiluted samples are as follows: Human Plasma was 4×10^5fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 251.55 ug/mL in human plasma.

    • Linearity
      The concentrations of Human Prealbumin were measured and interpolated from the target standard curves and corrected for sample dilution.
      The sample is undiluted samples are as follows: HepG2 supernatant was 200fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 114.82 ng/mL in HepG2 supernatant.

    • Specificity
      Test the specificity of different serum.

    • Protocol Diagram

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