Rabbit lgG/lgM isotype OneStep ELISA Kit

The rabbit immune system generates antibody diversity and optimizes affinity by mechanisms different from those of mice and other rodents. Compared with traditional mouse mAbs, the rabbit mAbs have advantages in diagnostics with high affinity and specificity toward antigens, more diverse epitope recognition, and greatly improved response to small-size epitopes and mouse antigens. The rabbit immunoglobulin heavy chain (IGH) locus contains over 200 IGH variable germline genes, with over 50% have been found to be “non-functional”. Also, more than 50 IG Kappa V and 17 IG Lambda V functional genes were identified with genomic sequencing (IMGT database). The diversity of rabbit VH repertoire is more limited, and the V light (VL) repertoire is more diverse than that in mice and humans. Most rabbit antibodies are derived from the IGHV1 gene. In addition, the rabbit VL repertoire displays a larger complementarity-determining region (CDR)-L3 loop length than its human and mouse counterparts. Rabbit serum and plasma samples contain around 10 mg/ml of IgG. The use of hybridoma technology or other platforms (e.g., phase display, single B cell antibody technology) for the production of rabbit monoclonal antibodies has created the requirement for a fast and simple procedure for quantifying antibody production. Rabbit IgM is a pentameric glycoprotein (approximately 970 kDa, with 10 antigen-binding sites and stabilized by the J chain) that plays a critical role in the humoral immune response of rabbits. As the primary antibody produced during initial immune responses, it functions as a key first responder by binding and neutralizing multivalent antigens, efficiently activating the complement system, promoting phagocytosis, and agglutinating pathogens. Compared to IgG, rabbit IgM exhibits distinct characteristics, including a shorter half-life and limited tissue penetration. Due to its functional properties, it holds significant value in research applications such as antibody production, diagnostic methods including blood typing and infection detection, and emerging therapeutic strategies.