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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Mouse & Rat CCL17 (Serum & Plasma) OneStep ELISA Kit

价格 ￥4,800.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3094
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    CCL17

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Ms, Rt

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 3.9%；
      Inter-assay: 6.6%

    • Sample type

      Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      16.023 pg/mL

    • Range

      23.44 pg/mL – 1500 pg/mL

    • Recovery

      Serum: 88%
      Plasma: 91%

    • Assay time

      60 minutes

    • Species reactivity

      Ms; Rt
背景介绍

  • Mouse CCL17 (TARC) is a CC chemokine that acts as the primary ligand for CCR4, specifically recruiting Th2 cells and regulatory T cells (Tregs). Produced by dendritic cells and macrophages under inflammatory stimulation, it serves as a key mediator of type 2 immunity. Its elevated expression drives allergic pathologies such as asthma and atopic dermatitis by promoting leukocyte infiltration and Th2-mediated inflammation, making it both a biomarker and a promising therapeutic target in allergic disease research.

  • ELISA

    • To assess the linearity of the assay, The sample was spiked with high concentrations of Mouse CCL17 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • To assess the linearity of the assay, The sample was spiked with high concentrations of Mouse CCL17 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Protocol Diagram

    • Standard curve
      Example of Mouse CCL17 standard curve in Assay Diluent #12Y.

    • Linearity
      The concentrations of Mouse CCL17 were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 145.98 pg/mL in Mouse serum.
      #2 sample is undiluted samples are as follows: The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 311.05 pg/mL in Rat serum.

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