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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Human & Monkey PSA OneStep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3091
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    PSA

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu, Mk

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 2.7%；
      Inter-assay: 5.3%

    • Sample type

      Cell culture supernatant; Serum; Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      7.291 pg/mL

    • Range

      31.25 pg/mL – 2000 pg/mL

    • Recovery

      Cell culture supernatant: 92%
      Serum: 93%
      Plasma: 113%

    • Assay time

      60 minutes

    • Species reactivity

      Hu; Mk
背景介绍

  • Human PSA, or Prostate-Specific Antigen, is a protein enzyme produced primarily by the cells of the prostate gland. Its primary biological function is to liquefy semen following ejaculation, which is crucial for sperm mobility and fertility. While most PSA is found in seminal fluid, a very small amount naturally leaks into the bloodstream. Measuring this serum PSA level through a blood test is a common tool for screening and monitoring prostate health. Elevated levels in the blood can indicate various conditions, including benign prostatic hyperplasia (BPH), prostatitis, or prostate cancer. It is important to note that PSA is not a perfect test for cancer, as levels can be raised by non-cancerous causes. Therefore, it is used alongside other diagnostic measures, such as the free PSA ratio, digital rectal exams, and imaging, to guide clinical decisions.

  • ELISA

    • Standard curve
      Example of Human PSA standard curve in Assay Diluent #1.

    • Linearity
      The concentrations of Human PSA were measured and interpolated from the target standard curves and corrected for sample dilution.
      Sample is undiluted samples are as follows: Human serum was 2fold dilution (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 748.94 pg/mL in human serum.

    • Linearity
      The concentrations of Human PSA were measured and interpolated from the target standard curves and corrected for sample dilution.
      Sample is undiluted samples are as follows: Human plasma was 2fold dilution (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1543.82 pg/mL in human plasma.

    • Linearity
      The concentrations of Human PSA were measured and interpolated from the target standard curves and corrected for sample dilution.
      Sample is undiluted samples are as follows: Monkey serum was 2fold dilution (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 2739.48 pg/mL in Monkey serum.

    • To assess the linearity of the assay, sample was spiked with high concentrations of Human PSA in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • To assess the linearity of the assay, two samples were spiked with high concentrations of Human PSA in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Leading Competitor comparison

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