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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Mouse IL-5(Culture Medium) OneStep ELISA Kit

货号： S0C3081

Datasheet COA
  • 价格： ￥3,000
  • 规格：
  • 数量：
斯达特大包装询价 大包装询价

产品介绍 评论(0)

产品规格

  • 抗原名称

    IL-5

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}, {"application": "ChIP", "dilution": "", "species": ""}]
试剂盒参数

    • Precision

      Intra-assay: 5.1%；
      Inter-assay: 6.1%

    • Sample type

      Cell culture supernatant

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.788 pg/mL

    • Range

      1.95 pg/mL – 125 pg/mL

    • Recovery

      Cell culture supernatant: 90%

    • Assay time

      60 minutes

    • Species reactivity

      Ms
背景介绍

  • Mouse Interleukin-5 (IL-5) is a Th2-derived cytokine critical for eosinophil biology. Produced primarily by activated T helper 2 cells and group 2 innate lymphoid cells (ILC2s) in mice, it signals through a heterodimeric receptor (IL-5Rα/βc) to regulate eosinophil differentiation, survival, and activation. This 45-kDa homodimeric glycoprotein drives allergic inflammation and anti-helminth immunity by promoting eosinophil migration to tissues and stimulating immunoglobulin production in B-1 cells. Its pivotal role in eosinophil-mediated pathologies makes it a key therapeutic target in murine asthma models and parasitic infections.

  • ELISA

    • Standard curve
      Example of Mouse IL-5 standard curve in Assay Diluent #1.

    • Linearity
      The concentrations of Mouse IL-5 were measured and interpolated from the target standard curves and corrected for sample dilution.
      The sample is undiluted samples are as follows: EL-4 cells stimulated with 10ug/ml PHA and 10ug/ml PMA for 2days (5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 4497.53 pg/mL in stimulated EL-4 cells supernatant.

    • The sample is undiluted samples are as follows: EL-4 cells culture for 2days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 87.39 pg/mL in unstimulated EL-4 supernatant.

    • To assess the linearity of the assay, The sample was spiked with high concentrations of Mouse IL-5 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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