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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Human IL-4R OneStep ELISA Kit

价格 ￥3,000.00 （ 1-2周）
货号 S0C3080
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IL-4R

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 4.5%；
      Inter-assay: 6.1%

    • Sample type

      Cell culture supernatant; Serum; Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      1.053 pg/mL

    • Range

      2.34 pg/mL – 150 pg/mL

    • Recovery

      Cell culture supernatant: 91%
      Serum: 103%
      Plasma: 103%

    • Assay time

      60 minutes

    • Species reactivity

      Hu
背景介绍

  • Human IL-4R (Interleukin-4 Receptor) is a critical cell surface receptor involved in immune regulation and inflammatory responses, composed of the IL-4Rα chain (CD124) paired with either the common γ-chain (γc, CD132) or IL-13Rα1 to form Type I or Type II receptors. It binds cytokines IL-4 and IL-13, activating JAK-STAT signaling pathways that modulate immune cell functions, particularly in hematopoietic cells such as B cells, T cells, macrophages, and mast cells. IL-4R signaling promotes Th2 cell differentiation, IgE antibody class switching, and anti-inflammatory responses, playing a central role in allergic and immune-mediated diseases. Dysregulation of IL-4R is associated with conditions like asthma, atopic dermatitis, and autoimmune disorders. Due to its therapeutic potential, IL-4R has been successfully targeted by drugs such as dupilumab, offering effective treatment for allergic diseases. Ongoing research continues to explore IL-4R's broader roles in immunity and inflammation, paving the way for novel therapies targeting immune dysregulation.

  • ELISA

    • Standard curve
      Example of Human IL-4R standard curve in Assay Diluent #11DS.

    • Linearity
      The concentrations of Human IL-4R were measured and interpolated from the target standard curves and corrected for sample dilution.
      Sample is undiluted samples are as follows: human plasma (25%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 565.71 pg/mL in human plasma #1. The mean target concentration was determined to be 739.72 pg/mL in human plasma #2.

    • Linearity
      The concentrations of Human IL-4R were measured and interpolated from the target standard curves and corrected for sample dilution.
      Sample is undiluted samples are as follows: human serum (25%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 464.86 pg/mL in human serum #1. The mean target concentration was determined to be 472.25 pg/mL in human serum #2.

    • Demonstration of specificity in mice
      The sample dilution factor was 4-fold.
      The added human IL-4R was detected in mouse serum, with a recovery rate of 101%.

    • Leading Competitor comparison

    • Protocol Diagram

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