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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Human GDF-15(Culture Medium) OneStep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3079
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    GDF-15

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 5.9%；
      Inter-assay: 9.4%

    • Sample type

      Cell culture supernatant

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.997 pg/mL

    • Range

      3.906 pg/mL – 250 pg/mL

    • Recovery

      Cell culture supernatant: 94%

    • Assay time

      60 minutes

    • Species reactivity

      Hu
背景介绍

  • Human Growth Differentiation Factor 15 (GDF-15), also known as MIC-1 or PDF, is a stress-responsive cytokine belonging to the TGF-β superfamily. Primarily secreted by macrophages, epithelial cells, and placental tissues under pathological conditions, this 25-kDa dimeric protein signals through the glial cell-derived neurotrophic factor (GDNF) family receptor α-like (GFRAL)-RET complex in the brainstem. It regulates systemic energy homeostasis by suppressing appetite and modulating insulin sensitivity, while also functioning as a biomarker for cellular stress, cardiovascular disease, and cancer progression. Elevated GDF-15 levels correlate with cachexia, heart failure, and tumor metastasis, making it a therapeutic target for metabolic disorders and a diagnostic indicator for tissue damage. Its expression increases >100-fold during starvation, inflammation, and oxidative stress.

  • ELISA

    • Standard curve
      Example of Human GDF-15 standard curve in Assay Diluent #1.

    • Linearity
      The concentrations of Human GDF-15 were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: HepG2 Cells culture was 3×10^2fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 87.32 ng/mL in HepG2 Cells culture

    • To assess the linearity of the assay, The samples was spiked with high concentrations of Human GDF-15 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Leading Competitor comparison

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