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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Human TNF-β OneStep ELISA kit

价格 ￥2,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3078
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    TNF-β

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 5.7%；
      Inter-assay: 7.5%

    • Sample type

      Cell culture supernatant, Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.110 ng/mL

    • Range

      0.156 pg/mL – 10 ng/mL

    • Recovery

      Cell culture supernatant: 86%
      Serum: 111%
      Plasma: 115%

    • Assay time

      60 minutes

    • Species reactivity

      Hu
背景介绍

  • Human Tumor Necrosis Factor-beta (TNF-β), also known as lymphotoxin-alpha (LT-α), is a pro-inflammatory cytokine primarily secreted by activated lymphocytes, including T cells, B cells, and NK cells. It signals through the TNF receptor 1 (TNFR1) and TNFR2, activating downstream pathways such as NF-κB, MAPK, and apoptosis cascades, which regulate immune responses and inflammation. Unlike TNF-α, which is produced by macrophages and other innate immune cells, TNF-β plays a specialized role in lymphoid organ development, host defense, and autoimmune pathogenesis. It contributes to the formation of secondary lymphoid structures and promotes cytotoxic T cell and B cell functions. Dysregulated TNF-β expression is associated with chronic inflammatory diseases, including rheumatoid arthritis and multiple sclerosis, making it a potential therapeutic target. Its dual role in immune regulation and pathology underscores its significance in both physiological and disease contexts.

  • ELISA

    • Standard curve
      Example of Human TNF-β standard curve in Assay Diluent #12.

    • Linearity
      The concentrations of Human TNF-β were measured and interpolated from the target standard curves and corrected for sample dilution.
      The sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be1.69 ng/mL in stimulated human PBMC supernatant.

    • To assess the linearity of the assay, the samples was spiked with high concentrations of Human TNF-β in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • To assess the linearity of the assay, two samples were spiked with high concentrations of Human TNF-β in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Protocol Diagram

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