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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Mouse IL-13 OneStep ELISA kits

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3077
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IL-13

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 3.4%；
      Inter-assay: 4.9%

    • Sample type

      Cell culture supernatant, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      2.413 pg/mL

    • Range

      15.63 pg/mL – 1000 pg/mL

    • Recovery

      Cell culture supernatant: 108%
      Plasma: 98%

    • Assay time

      60 minutes

    • Species reactivity

      Ms
背景介绍

  • Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. The secondary structural features of IL-13 are similar to that of Interleukin 4 (IL-4); however it only has 25% sequence identity to IL-4 and is capable of IL-4 independent signaling. IL-13 is a cytokine secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosinophils and nuocytes. Interleukin-13 is a central regulator in IgE synthesis, goblet cell hyperplasia, mucus hypersecretion, airway hyperresponsiveness, fibrosis and chitinase up-regulation. It is a mediator of allergic inflammation and different diseases including asthma.

  • ELISA

    • Standard curve
      Example of Mouse IL-13 standard curve in Assay Diluent #12Y.

    • Linearity
      The concentrations of Mouse IL-13 were measured and interpolated from the target standard curves and corrected for sample dilution.
      The sample is undiluted samples are as follows: EL-4 cells stimulated with 10ug/ml PHA and 10ug/ml PMA for 2days (20%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 5348.63 pg/mL in stimulated EL-4 cells supernatant.

    • To assess the linearity of the assay, the samples was spiked with high concentrations of Mouse IL-13 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • To assess the linearity of the assay, the samples was spiked with high concentrations of Mouse IL-13 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Leading Competitor comparison

    • Protocol Diagram

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