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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Human Lysozyme OneStep ELISA kits

价格 ￥3,000.00 （ 1-2周）
货号 S0C3074
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    Lysozyme

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 4.3%；
      Inter-assay: 6.4%

    • Sample type

      Cell culture supernatant, Serum,Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      2.167 pg/mL

    • Range

      15.63 pg/mL – 1000 pg/mL

    • Recovery

      Cell culture supernatant: 87%
      Serum: 86%
      Plasma: 102%

    • Assay time

      60 minutes

    • Species reactivity

      Human
背景介绍

  • Human lysozyme (LZM/LYZ), a 14.4-kDa bacteriolytic enzyme encoded by the LYZ gene on chromosome 12, functions as a critical innate immune defender by hydrolyzing β-1,4-glycosidic bonds in peptidoglycan of bacterial cell walls. Primarily secreted by neutrophils, macrophages, and epithelial cells, it is abundantly present in secretions (tears, saliva) and biological fluids, with serum/plasma concentrations typically ranging from 1.5–3.0 μg/mL in healthy adults. Clinically, elevated serum levels (>5 μg/mL) serve as a biomarker for bacterial infections (e.g., tuberculosis, meningitis), monocytic leukemia (due to malignant cell release), and renal tubular dysfunction (reduced reabsorption), while decreased levels correlate with immunosuppression. Detection relies on high-sensitivity ELISA (limit: ~0.5 ng/mL), though sample handling requires rigorous centrifugation and -80°C storage to prevent degradation. Its dual role as an antimicrobial agent and disease indicator underscores its significance in both physiological immunity and pathological diagnostics.

  • ELISA

    • Standard curve
      Example of Human lysozyme standard curve in Assay Diluent #1.

    • Linearity
      The concentrations of Human lysozyme were measured and interpolated from the target standard curves and corrected for sample dilution.
      The sample is undiluted samples are as follows: Human serum was 2X10^3 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 724.69 ng/mL in human serum.

    • Linearity
      The sample is undiluted samples are as follows: Human plasma was 2X10^3fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 594.69 ng/mL in human plasma.

    • To assess the linearity of the assay,two samples were spiked with high concentrations of Human Lysozyme in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • To assess the linearity of the assay,the sample was spiked with high concentrations of Human Lysozyme in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Leading Competitor comparison

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