Mouse IL-4(Cell culture supernatant) OneStep ELISA Kit

Datasheet COA

价格￥3,000.00 （现货： 2-5个工作日）

货号 S0C3073

规格

1x96T5x96T10x96T

数量

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产品规格

抗原名称

IL-4
免疫原

Recombinant Protein
抗体类型

Recombinant mAb
反应种属 ?

Ms
纯化方式

Protein A
储存条件

12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

- Precision
  
  Intra-assay: 3.3%；
  Inter-assay: 3.7%
- Sample type
  
  Cell culture supernatant
- Assay type
  
  Sandwich (quantitative)
- Sensitivity
  
  1.004 pg/mL
- Range
  
  4.688 pg/mL – 300 pg/mL
- Recovery
  
  Cell culture supernatant: 91%
- Assay time
  
  60 minutes
- Species reactivity
  
  Ms

背景介绍

Mouse interleukin-4 (IL-4) is an 18–20 kDa monomeric glycoprotein cytokine primarily produced by Th2 cells, mast cells, basophils, eosinophils, NKT cells, and γδT cells. As a central regulator of type 2 immunity, it drives Th2 differentiation via the JAK-STAT6 pathway upon binding to IL-4 receptor complexes (IL-4Rα paired with γc or IL-13Rα1), inducing transcription factors like GATA3 and c-Maf. Functionally, IL-4 promotes B cell proliferation, class switching to IgG1/IgE, and activation of eosinophils/mast cells, while suppressing Th1 responses. Its roles are context-dependent: IL-4 exerts protective effects in acute lung injury (reducing TNF-α/IL-6 and apoptosis) and cerebral ischemia (attenuating neuroinflammation), yet can exacerbate infections (e.g., mousepox) by suppressing cytolytic lymphocyte responses.

ELISA
- Standard curve
  Example of Mouse IL-4 standard curve in Assay Diluent #1.
- Linearity
  The concentrations of Mouse IL-4 were measured and interpolated from the target standard curves and corrected for sample dilution.
  The sample is undiluted samples are as follows: EL-4 cells stimulated with 10ug/ml PHA and 10 ug/ml PMA for 2 days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 290.27 pg/mL in stimulated EL-4 cells supernatant.
- Linearity
  The sample is undiluted samples are as follows: EL-4 cells culture for 2 days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 64.18 pg/mL in unstimulated EL-4 cells supernatant.
- Linearity
  The concentrations of Mouse IL-4 were measured and interpolated from the target standard curves and corrected for sample dilution.
  #1 sample is undiluted samples are as follows: Mouse splenocyte were cultured for 3days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 12.23 pg/mL in Mouse splenocytes culture supernatant.
  #2 sample is undiluted samples are as follows: An aliquot of the culture medium was removed (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3.89 pg/mL in culture medium.
- To assess the linearity of the assay, sample was spiked with high concentrations of Mouse IL-4 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
- Leading Competitor comparison