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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Human IL-7 OneStep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3071
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IL-7

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 3.7%；
      Inter-assay: 6.0%

    • Sample type

      Cell culture supernatant, Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.653 pg/ml

    • Range

      1.56 pg/mL – 100 pg/mL

    • Recovery

      Cell culture supernatant: 115%
      Serum: 98%
      Plasma: 114%

    • Assay time

      60 minutes

    • Species reactivity

      Human
背景介绍

  • IL7, also known as interleukin 7, is a hematopoietic growth factor that belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells, but is not produced by lymphocytes. Human IL‑7 cDNA encodes 177 amino acids (aa) that include a 25 aa signal peptide. Human IL-7 shares approximately 60-63% aa sequence identity with mouse, rat, canine and feline IL-7, and 72-76% with equine, bovine, ovine, and porcine IL-7. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Like other common gamma-chain cytokines like IL-2 and IL-15, IL-7 and its receptor, IL-7R, have been used in a variety of immunotherapy applications, often in fluid tumors and in some instances of solid tumor models.

  • ELISA

    • Standard curve
      Example of Human IL-7 standard curve in Assay Diluent #11Y.

    • Linearity
      The concentrations of Human IL-7 were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: Human Serum was 4 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 11.90 pg/mL in Human Serum.
      #2 sample is undiluted samples are as follows: Human Plasma was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3.19 pg/mL in Human Plasma.

    • To assess the linearity of the assay, three samples were spiked with high concentrations of Human IL-7 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Leading Competitor comparison

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