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抗原名称
IGF-1免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Hu纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
Precision
Intra-assay: 4.2%；
Inter-assay: 7.7%Sample type
Cell culture supernatant, Serum, PlasmaAssay type
Sandwich (quantitative)Sensitivity
0.054 ng/mlRange
0.47 ng/mL – 30 ng/mLRecovery
Cell culture supernatant: 99%
Serum: 106%
Plasma: 110%Assay time
60 minutesSpecies reactivity
Human
Human Insulin-like Growth Factor-1 (IGF-1) is a 7.6 kDa polypeptide hormone structurally similar to insulin, playing a crucial role in growth, development, and metabolic regulation. Primarily produced in the liver under stimulation by growth hormone (GH), IGF-1 mediates many of GH's anabolic and mitogenic effects via activation of the IGF-1 receptor (IGF-1R), a tyrosine kinase receptor that triggers downstream signaling pathways such as PI3K/AKT and MAPK/ERK. IGF-1 is essential for: Growth & Development: Promotes cellular proliferation, differentiation, and tissue growth, particularly in bone, muscle, and nervous systems. Metabolic Regulation: Enhances glucose uptake, protein synthesis, and inhibits apoptosis. Tissue Repair: Supports regeneration in muscle, cartilage, and neuronal tissues. Clinically, recombinant human IGF-1 (rhIGF-1, e.g., mecasermin) is used to treat growth disorders like Laron syndrome and severe primary IGF-1 deficiency. It is also investigated for conditions such as diabetes, neurodegenerative diseases, and muscle wasting disorders. However, its dysregulation is associated with cancer progression and acromegaly.
ELISA
Standard curve
Example of Human IGF-1 standard curve in Assay Diluent #8.Linearity
The concentrations of Human IGF-1 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: Human serum(10%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 123.69ng/mL in human serum.Linearity
The concentrations of Human IGF-1 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: Human plasma(10%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 183.03ng/mL in human serum.
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