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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Human IgG (Total) OneStep ELISA Kit (broad range)

价格 ￥3,000.00 （现货 ： 2-5个工作日）
货号 S0C3066
规格
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产品介绍 评论(0)

产品规格

  • 抗原名称

    IgG (Total)

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}, {"application": "ChIP", "dilution": "", "species": ""}]
试剂盒参数

    • Precision

      Intra-assay: 4.6%；
      Inter-assay: 6.3%

    • Sample type

      Cell culture supernatant, Serum,Plasma

    • Assay type

      competitive

    • Sensitivity

      0.043 ug/ml

    • Range

      0.41 ug/mL – 100 ug/mL

    • Recovery

      Cell culture supernatant: 102%
      Serum: 104%
      Plasma: 105%

    • Assay time

      60 minutes

    • Species reactivity

      Human
背景介绍

  • Immunoglobulin G (IgG) is a type of antibody. IgG is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This kit is for the quantitative measurement of human total IgG in Human serum, plasma and supernatant from cell cultures.

  • ELISA

    • Standard curve
      Example of Human IgG standard curve in Assay Diluent #1.

    • Linearity
      The concentrations of Human IgG were measured and interpolated from the target standard curves and corrected for sample dilution.
      The sample is undiluted samples are as follows: Human Serum was 2.5×10^2fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 11.71 mg/mL in Human Serum.

    • The sample is undiluted samples are as follows: Human Plasma was 100fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 8.72 mg/mL in Human Plasma.

    • Protocol Diagram

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