产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
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抗原名称
PCSK9免疫原
Recombinant Protein抗体类型
Recombinant mAb纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8'C as supplied.
Precision
Intra-assay: 4.3%；
Inter-assay: 5.2%Sample type
Cell culture supernatant, Serum, PlasmaAssay type
Sandwich (quantitative)Sensitivity
11.177 pg/mlRange
31.25 pg/mL – 2000 pg/mLRecovery
Cell culture supernatant: 85%
Serum: 100%
Plasma: 87%Assay time
60 minutesSpecies reactivity
Human; Monkey
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a key regulator of cholesterol metabolism, primarily known for its role in degrading low-density lipoprotein receptors (LDLR) in the liver. By binding to LDLR and promoting its lysosomal degradation, PCSK9 reduces the liver's ability to clear circulating LDL cholesterol (LDL-C), leading to elevated plasma LDL-C levels. Gain-of-function mutations in the PCSK9 gene are associated with autosomal dominant hypercholesterolemia, while loss-of-function variants correlate with reduced LDL-C and cardiovascular risk.
ELISA
Standard curve
Example of Human PCSK9 standard curve in Assay Diluent #1.Linearity
The concentrations of Human PCSK9 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: HepG2 human hepatocellular carcinoma cells culture supernatant (5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 25.61 ng/mL in HepG2 cells culture supernatant.Linearity
The concentrations of Human PCSK9 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human serum (1%) and human plasma (1%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 465.62 ng/mL in human serum. The mean target concentration was determined to be 482.06 ng/mL in human serum .Linearity
The concentrations of Human PCSK9 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: female monkey serum (2%) and male monkey serum (2%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 91.66 ng/mL in female monkey serum. The mean target concentration was determined to be 98.79 ng/mL in male monkey serum.Specificity
Test the specificity of different serum.To assess the linearity of the assay, sample was spiked with high concentrations of Human PCSK9 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay, two samples were spiked with high concentrations of Human PCSK9 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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