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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Human Thyroid Stimulating Hormone OneStep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3062
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    Thyroid Stimulating Hormone

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 3.6%；
      Inter-assay:4.4%

    • Sample type

      Cell culture supernatant, Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      2.135 pg/ml

    • Range

      7.81 pg/mL – 500 pg/mL

    • Recovery

      Cell culture supernatant: 111%
      Serum: 100%
      Plasma: 102%

    • Assay time

      60 minutes

    • Species reactivity

      Human
背景介绍

  • Human Thyroid Stimulating Hormone (TSH), a glycoprotein hormone secreted by the anterior pituitary gland, plays a pivotal role in regulating thyroid function by stimulating the synthesis and secretion of thyroid hormones (T3 and T4), which are essential for metabolism, growth, and development. TSH binds to TSH receptors on thyroid follicular cells and its secretion is regulated by a negative feedback loop involving thyrotropin-releasing hormone (TRH) from the hypothalamus and circulating T3/T4 levels. As the most sensitive marker for thyroid disorders, elevated TSH indicates hypothyroidism while suppressed TSH suggests hyperthyroidism. With a molecular weight of ~28 kDa consisting of α- and β-subunits, TSH is typically measured using immunoassays (ELISA, CLIA, RIA) with a normal range of 0.4-4.0 mIU/L. Clinically, TSH testing is indispensable for diagnosing various thyroid conditions (Hashimoto's thyroiditis, Graves' disease), monitoring levothyroxine therapy, and assessing thyroid function during pregnancy, making it the cornerstone of thyroid function evaluation due to its high diagnostic reliability.

  • ELISA

    • Standard curve
      Example of Human TSH standard curve in Assay Diluent #12Y.

    • Standard curve
      Example of Human TSH standard curve in Assay Diluent #12Y.

    • Linearity
      The concentrations of TSH were measured and interpolated from the target standard curves and corrected for sample dilution.
      The sample is undiluted samples are as follows: Human serum was 16fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 187.67 pg/mL in human serum.

    • The sample is undiluted samples are as follows: Human plasma was 2fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 168.60 pg/mL in human plasma.

    • To assess the linearity of the assay, two samples were spiked with high concentrations of Human TSH in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • To assess the linearity of the assay, The sample was spiked with high concentrations of Human TSH in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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