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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Rat IGF-1 OneStep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3053
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IGF-1

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Rt

  • 纯化方式

    Protein A

  • 储存条件

    2 to 8 °C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 5.6%；
      Inter-assay: 7.2%

    • Sample type

      Cell culture supernatant, Serum

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      4 pg/ml

    • Range

      6.25 pg/mL – 400 pg/mL

    • Recovery

      Cell culture supernatant: 106%
      Serum: 96%
      Plasma: 115%

    • Assay time

      60 minutes

    • Species reactivity

      Rat
背景介绍

  • Rat Insulin-like Growth Factor-1 (IGF-1) is a key regulatory protein involved in growth, metabolism, and cellular repair. Structurally homologous to human and mouse IGF-1, it binds to the IGF-1 receptor (IGF-1R) to activate downstream signaling pathways such as PI3K/Akt and MAPK, promoting cell proliferation, differentiation, and survival. Rat IGF-1 is widely utilized in research to investigate growth disorders, metabolic diseases, tissue regeneration, and aging processes in rat models. Recombinant rat IGF-1, produced in E. coli or mammalian expression systems, ensures high biological activity and consistency for experimental applications. It is particularly valuable in studies related to diabetes, neuroprotection, muscle development, and wound healing.

  • ELISA

    • Standard curve
      Example of Rat IGF-1 standard curve in Assay Diluent #5.

    • Linearity
      The concentrations of Rat IGF-1 were measured and interpolated from the target standard curves and corrected for sample dilution.
      Sample is undiluted samples are as follows: Rat Serum was 1x10^3 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 692.1 ng/mL in Rat Serum.

    • To assess the linearity of the assay, sample was spiked with high concentrations of Rat IGF-1 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • To assess the linearity of the assay, two samples were spiked with high concentrations of Rat IGF-1 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Leading Competitor comparison

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