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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Rat IFN-γ OneStep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3052
规格
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产品介绍 评论(0)

产品规格

  • 抗原名称

    IFN-γ

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Rt

  • 纯化方式

    Protein A

  • 储存条件

    2 to 8 °C as supplied.

  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}, {"application": "ChIP", "dilution": "", "species": ""}]
试剂盒参数

    • Precision

      Intra-assay: 3.1%；
      Inter-assay: 3.9%

    • Sample type

      Cell culture supernatant, Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.846 pg/ml

    • Range

      2.34 pg/mL – 150 pg/mL

    • Recovery

      Cell culture supernatant: 92%
      Serum: 81%
      Plasma: 101%

    • Assay time

      60 minutes

    • Species reactivity

      Rat
背景介绍

  • Interferon gamma (IFNG or IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. Through cell signaling, interferon gamma plays a role in regulating the immune response of its target cell. A key signaling pathway that is activated by type II IFN is the JAK-STAT signaling pathway. IFNG plays an important role in both innate and adaptive immunity. Type II IFN is primarily secreted by adaptive immune cells, more specifically CD4+ T helper 1 (Th1) cells, natural killer (NK) cells, and CD8+ cytotoxic T cells. The expression of type II IFN is upregulated and downregulated by cytokines. By activating signaling pathways in cells such as macrophages, B cells, and CD8+ cytotoxic T cells, it is able to promote inflammation, antiviral or antibacterial activity, and cell proliferation and differentiation. Type II IFN has played a role in the development of cancer immunotherapy treatments due to its ability to prevent tumor growth.

  • ELISA

    • Standard curve
      Example of Rat IFN-γ standard curve in Assay Diluent #1.

    • Standard curve
      Example of Rat IFN-γ standard curve in Assay Diluent #1R1000.

    • Linearity
      The concentrations of Rat IFN-γ were measured and interpolated from the target standard curves and corrected for sample dilution.
      The sample is undiluted samples are as follows: Rat splenocyte cells stimulated with 5 µg/mL of Concanavalin A for 48 hours （1.25%）. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 5999.01 pg/mL in stimulated Rat splenocyte cells.

    • Linearity
      The sample is undiluted samples are as follows: Rat splenocyte cells culture for 48 hours （50%）. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 573.93 pg/mL in unstimulated Rat splenocyte cells.

    • To assess the linearity of the assay, in cell culture media with high concentrations of Rat IFN-γ in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • To assess the linearity of the assay, in serum and plasma with high concentrations of Rat IFN-γ in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Leading Competitor comparison

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