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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Mouse IGF-1 OneStep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3051
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产品介绍 评论(0)

产品规格

  • 抗原名称

    IGF-1

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein A

  • 储存条件

    2 to 8 °C as supplied.

  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}, {"application": "ChIP", "dilution": "", "species": ""}]
试剂盒参数

    • Precision

      Intra-assay: 4.7%；
      Inter-assay: 9.9%

    • Sample type

      Cell culture supernatant, Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      4.443pg/ml

    • Range

      6.25 pg/mL – 400 pg/mL

    • Recovery

      Cell culture supernatant: 103%
      Serum: 89%
      Plasma: 101%

    • Assay time

      60 minutes

    • Species reactivity

      Mouse
背景介绍

  • Mouse Insulin-like Growth Factor-1 (IGF-1) is a critical polypeptide hormone that plays a vital role in growth, development, and cellular metabolism. Structurally and functionally similar to human IGF-1, it mediates the effects of growth hormone (GH) by promoting proliferation, differentiation, and survival of various cell types. Mouse IGF-1 is widely used in biomedical research to study growth regulation, tissue repair, and metabolic processes in murine models. Its applications span areas such as endocrinology, cancer research, and regenerative medicine. Recombinant mouse IGF-1, produced via E. coli or mammalian expression systems, ensures high purity and bioactivity for experimental use.

  • ELISA

    • Standard curve
      Example of Mouse IGF-1 standard curve in Assay Diluent #5.

    • Linearity
      The concentrations of Mouse IGF-1 were measured and interpolated from the target standard curves and corrected for sample dilution.
      Sample is undiluted samples are as follows: Mouse Serum was 2x10^3 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 146.7 ng/mL in Mouse Serum.

    • To assess the linearity of the assay, sample was spiked with high concentrations of Mouse IGF-1 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • To assess the linearity of the assay, two samples were spiked with high concentrations of Mouse IGF-1 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Leading Competitor comparison

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