产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Rat IgA OneStep ELISA Kit

货号: S0C3039

  • 价格: ¥3,200
  • 规格:
  • 数量:
斯达特大包装询价 大包装询价

产品介绍 评论(0)

产品规格
  • 抗原名称

    IgA
  • 免疫原

    Recombinant Protein
  • 抗体类型

    Recombinant mAb
  • 反应种属 ?

    Rt
  • 纯化方式

    Protein A
  • 储存条件

    2 to 8 °C as supplied.
  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}]
试剂盒参数
    • Precision

      Intra-assay: 5.7%;
      Inter-assay: 7.1%
    • Sample type

      Cell culture supernatant, Serum, Plasm
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      0.043 ng/ml
    • Range

      1.56 ng/mL – 100ng/mL
    • Recovery

      Cell culture supernatant: 99%
      Serum: 90%
      Plasm: 115%
    • Assay time

      60 minutes
    • Species reactivity

      Rat
背景介绍
  • Immunoglobulin A (IgA) is a pivotal antibody isotype central to mucosal immunity and systemic immune regulation in rats. Structurally, rat IgA exists predominantly as a monomer in serum and as a dimer or polymer (secretory IgA, sIgA) at mucosal surfaces. Each IgA molecule comprises two α-heavy chains and two light chains (κ or λ), forming a flexible Y-shaped structure stabilized by disulfide bonds. The α-heavy chain contains three constant domains (Cα1–Cα3), with the Fc region (Cα2–Cα3) facilitating interactions with the Fcα receptor (FcαRI) and the polymeric immunoglobulin receptor (pIgR). Secretory IgA incorporates a J-chain and a secretory component, which enhance its stability in protease-rich mucosal environments. Extensive glycosylation of rat IgA further modulates its solubility, receptor binding, and resistance to enzymatic degradation. Rat IgA is the primary antibody safeguarding mucosal surfaces (e.g., respiratory, gastrointestinal tracts), where it neutralizes pathogens, blocks microbial adhesion, and maintains commensal microbiota balance. Secretory IgA mediates immune exclusion by trapping pathogens in mucus for mechanical clearance, while serum IgA contributes to systemic immune homeostasis by regulating inflammatory responses via FcαRI signaling. Additionally, rat IgA may engage in eosinophil-mediated antiparasitic defense and modulate allergic or autoimmune reactions through interactions with immune cells. Rat IgA shares conserved structural motifs with murine and human IgA, particularly in the α-chain constant domains. However, species-specific variations in glycosylation patterns, J-chain assembly, and secretory component binding necessitate species-specific assays for accurate detection. For example, rat IgA lacks the extended hinge region characteristic of human IgA1, resulting in distinct antigen accessibility and protease resistance. Clinical and Experimental Significance of IgA Levels: Elevated IgA: Associated with chronic mucosal infections (e.g., intestinal pathogens), autoimmune disorders (e.g., experimental colitis models), or inflammatory conditions. Reduced IgA: May indicate primary immunodeficiency (e.g., selective IgA deficiency in genetically modified models), immunosuppressive therapies, or gut dysbiosis. Applications of the Rat IgA ELISA Kit: This OneStep Rat IgA ELISA kit is designed for rapid and precise quantification of IgA in serum, plasma, or cell culture supernatants. Advantages of this OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 1 hours, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable IgA quantification in time-sensitive or resource-limited settings.

  • ELISA

    • Standard curve
      Example of Rat IgA standard curve in Assay Diluent #1.

    • #2 sample is undiluted samples are as follows: Rat Plasma was 1.0×10^3fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 39.34 μg/mL in Rat Plasma.

    • To assess the linearity of the assay, in cell culture media with high concentrations of Rat IgA in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • To assess the linearity of the assay, in serum and plasma with high concentrations of Rat IgA in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Specificity
      Test the different specificity of IgA standards and serum: sample #1 is Mouse IgA (100 ng/mL); sample #2 is Rat IgA (100 ng/mL); sample #3 is Human IgA (100 ng/mL). sample #4 is Mouse serum; sample #5 is Rat serum; sample #6 is Human serum; sample #7 is blank.

    • Protocol Diagram

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