产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
大包装询价 产品介绍 评论(0)
抗原名称
Protein A免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Others储存条件
2 to 8 °C as supplied.
Precision
板内变异系数 (Intra-assay): 4.7%；
板间变异系数 (Inter-assay): 6.3%Sample type
Cell culture supernatantAssay type
Sandwich (quantitative)Sensitivity
8.910 pg/mlRange
39.06 pg/mL – 2500 pg/mLRecovery
Rabbit IgG: 89%
Human IgG: 110%
Mouse IgG: 113%Assay time
60 minutes
Protein A is a bacterial cell wall protein, originally derived from Staphylococcus aureus, that exhibits a high affinity for the Fc region of immunoglobulins (IgG). It is widely used in various applications, such as antibody purification, immunoassays, protein-protein interaction studies, and therapeutic antibody development. Protein A residues can potentially be present in biopharmaceutical products as a result of the purification process using Protein A affinity chromatography. The presence of Protein A residues in the final product is a concern due to their potential immunogenicity and safety implications. Therefore, the detection and quantification of Protein A residues in biopharmaceutical products is crucial for ensuring product quality and patient safety. This simple, sensitive, and fast OneStep ELISA is a powerful method for optimizing purification processes, process control, routine quality control, and product release testing.
ELISA
Standard curve
Example of Protein A standard curve in Assay Diluent #8(used Treatment 1).Standard curve
Example of Protein A standard curve in Assay Diluent #8(used Treatment 2).Linearity
The concentrations of Protein A were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: Rabbit monoclonal antibody sample based on a 100ug/mL antibody load (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1.3 ng/mL Rabbit monoclonal antibody sample.Calibration
Test the Protein A standard and domain as follows: B domain of Protein A were calibrated by Protein A standard proteins with a calibration factor (CF) of 0.5. B domain of Protein A concentration = Protein A standard protein concentration/CFCalibration
Protein A mutant Z domain were calibrated by Protein A standard proteins with a calibration factor (CF) of 0.4;
Protein A mutant Z domain (A1V, N3A, N6D, N23T, G29A, P57I) were calibrated by Protein A standard proteins with a calibration factor (CF) of 0.2. Protein A mutant concentration = Protein A standard protein concentration/CFSpecificity
Simulation Test for the Shedding of Protein A agarose
#1 sample is cytiva MabSelect PrismA(Cat：17549801): C#1, C#2, C#3 and C#4 were treated under different conditions to detect Protein A. C#1 were treated with descaling agents and strong acids; C#2 treated with descaling agents, strong acid and heat; C#3 were treated with descaling agents, strong acids and ultracentrifugation; C#4 treated with descaling agents, strong acids, heat and ultracentrifugation.Specificity
#2 sample is GenScript Monofinity A Resin (Cat. No: L00433-500): G#1, G#2, G#3 and G#4 were treated under different conditions to detect Protein A. G#1 were treated with descaling agents and strong acids; G#2 treated with descaling agents, strong acid and heat; G#3 were treated with descaling agents, strong acids and ultracentrifugation; G#4 treated with descaling agents, strong acids, heat and ultracentrifugation.Leading Competitor comparison
Protocol Diagram
评论(0)