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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Mouse IL-6 OneStep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3019-HL
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IL-6

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 2.8%；
      Inter-assay: 4.2%

    • Sample type

      Serum (Balb/c); Heparin Plasma (CD-1), Citrate Plasma (Balb/c, C57BL/6); Cell Culture Supernatant

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      2.87 pg/mL

    • Range

      7.8 pg/mL – 500 pg/mL

    • Recovery

      Serum (Balb/c): 106%
      Citrate plasma (Balb/c):113%
      Citrate plasma (C57BL/6): 93%
      Heparin Plasma (CD-1): 106%
      Cell Culture Supernatant: 111%

    • Assay time

      60 minutes

    • Species reactivity

      Ms
背景介绍

  • Mouse interleukin-6 (IL-6) is a pleiotropic cytokine encoded by the Il6 gene on chromosome 5, produced primarily by immune cells, fibroblasts, and endothelial cells in response to infection, injury, or inflammatory stimuli, and it exerts its biological effects through a receptor complex composed of IL-6Rα and gp130, activating downstream JAK/STAT3, MAPK, and PI3K signaling cascades that drive diverse functions including acute-phase protein synthesis in hepatocytes, Th17 cell differentiation, B-cell maturation, osteoclastogenesis, and metabolic regulation, while its dysregulation is linked to chronic inflammation and autoimmune pathology, as demonstrated by Il6-deficient mice which show reduced inflammatory responses and resistance to experimental autoimmune encephalomyelitis, highlighting its central role as both a protective mediator of host defense and a pathogenic driver in inflammatory disease models.

  • ELISA

    • Standard Curve
      Example of Mouse IL-6 standard curve in Assay Diluent EC8. Plotted are the background-subtracted data.
      This standard curve is provided for demonstration only.

    • Spike Recovery
      The recovery of Mouse IL-6 was evaluated in activated samples spiked with concentrations spanning the entire assay range.

    • Intra-Assay Precision (Precision within an assay)
      Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
      Inter-Assay Precision (Precision between assays).
      Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.

    • Determination of Minimum Detectable Dose (MDD)
      The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
      MDD = 2 × STDEV + AVERAGE

    • HOOK Effect Threshold
      The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.

    • Protocol Diagram

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