产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
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免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Hu纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
Precision
Intra-assay: 5.0%；
Inter-assay: 6.5%Sample type
Serum, PlasmaAssay type
Sandwich (quantitative)Sensitivity
1.335 pg/mlRange
7.8 pg/mL – 500 pg/mLRecovery
Serum: 105%
Plasma: 83%Assay time
60 minutesSpecies reactivity
Human
Interleukin-1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1βgene. IL-1β is a member of the interleukin 1 family of cytokines. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Increased production of IL-1β causes a number of different autoinflammatory syndromes, most notably the monogenic conditions referred to as Cryopyrin-Associated Periodic Syndromes (CAPS), due to mutations in the inflammasome receptor NLRP3 which triggers processing of IL-1β.
ELISA
Standard curve
Example of Human IL-1β standard curve in Assay Diluent #12E5.Linearity
The concentrations of Human IL-1β were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 942.47 pg/mL in stimulated human PBMC supernatant.Protocol Diagram
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