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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Human IL-5 OneStep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3009-HL
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IL-5

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 4.7%；
      Inter-assay: 6.4%

    • Sample type

      Cell culture supernatant; Serum; Plasma-Citrate; Plasma-EDTA

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      1.1 pg/mL

    • Range

      3.9 pg/mL – 250 pg/mL

    • Recovery

      Cell culture supernatant: 97%
      Serum: 76%
      Plasma-Citrate: 100%
      Plasma-EDTA: 96%

    • Assay time

      60 minutes

    • Species reactivity

      Hu

    • NIBSC convert

      The dose response curve of the International Standard (90/586) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (90/586) units, use the following equation: NIBSC (90/586) approximate value (IU/mL) = 0.01 x OneStep Human IL-5 value (pg/mL).
背景介绍

  • IL-5 protein, also known as Interleukin-5, is a protein molecule produced by the immune system and belongs to the category of cytokines. It is primarily expressed by T lymphocytes and other immune cells, such as NK cells. IL-5 plays a crucial role in regulating and mobilizing the body's immune response. IL-5 protein is critical for the generation, proliferation, and activation of eosinophils and basophils. These cells play significant roles in allergic diseases (such as allergic asthma and rhinitis) and certain parasitic infections. Given these characteristics of IL-5 protein, it has potential applications in in vitro diagnostics. By measuring the levels of IL-5 protein in bodily fluids, it can assist in diagnosing diseases associated with eosinophils or basophils, such as eosinophilic asthma and allergic rhinitis. These diseases are often linked to excessive expression or dysfunctional IL-5 protein. Furthermore, the level of IL-5 protein can reflect the state of the immune system.

  • ELISA

    • Standard curve
      Example of Human H-FABP standard curve in Assay Diluent DA4.

    • Linearity
      The concentrations of Human IL-5 were measured and interpolated from the target standard curves and corrected for sample dilution.
      The sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 178.95 pg/mL in stimulated human PBMC supernatant.

    • To assess the linearity of the assay, the sample was spiked with high concentrations of Human IL-5 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • To assess the linearity of the assay, two samples were spiked with high concentrations of Human IL-5 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Leading Competitor comparison

    • Protocol Diagram

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