产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
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抗原名称
IgG (Total)免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Hu纯化方式
Protein A储存条件
2 to 8 °C as supplied.
Precision
Intra-assay: 7.3%；
Inter-assay: 7.5%
Sample type
Cell culture supernatant, Serum, PlasmaAssay type
Sandwich (quantitative)Sensitivity
80 pg/mlRange
234 pg/ml - 15000 pg/mlRecovery
Cell culture supernatant 97%
Serum 88%
Plasma 93%Assay time
60 minutesSpecies reactivity
HumanCROSS REACTIVITY
/INTERFERENCE
/NIBSC convert
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Immunoglobulin G (IgG) is a type of antibody. IgG is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This kit is for the quantitative measurement of human total IgG in Human serum, plasma and supernatant from cell cultures.
ELISA
Standard curve
Example of Human IgG (Total) standard curve in Assay Diluent #1.Demonstration of the tested species specificity
Linearity
The concentrations of total IgG were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Human serum was 5×10^5 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 10mg/mL in human serum.
#2 sample is undiluted samples are as follows: Human plasma was 5×10^5 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 6.2 mg/mL in human plasma.Leading Competitor comparison
Protocol Diagram
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