Human & Monkey IL-6 OneStep ELISA Kit (High Level)

Datasheet COA

价格￥3,000.00 （供应商现货： 3-5个工作日）

货号 S0C3004-HL

规格

1x96T5x96T10x96T

数量

产品介绍评论(0)

产品规格

抗原名称

IL-6
免疫原

Recombinant Protein
抗体类型

Recombinant mAb
反应种属 ?

Hu, Mk
纯化方式

Protein A
储存条件

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

试剂盒参数

- Precision
  
  Intra-assay: 3.6%；
  Inter-assay: 7.2%
- Sample type
  
  Cell culture supernatant , Serum, Plasma
- Assay type
  
  Sandwich (quantitative)
- Sensitivity
  
  0.466 pg/mL
- Range
  
  2.1 pg/mL – 136 pg/mL
- Recovery
  
  Cell culture media: 88%
  Serum: 109%
  Plasma: 88%
- Assay time
  
  60 minutes
- Species reactivity
  
  Hu, Mk
- NIBSC convert
  
  The dose response curve of the International Standard (21/308) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (21/308) units, use the following equation: NIBSC (21/308) approximate value (IU/mL) = 0.142 x OneStep Human IL-6 value (pg/mL).

背景介绍

Interleukin 6 (IL-6) is a prototypical cytokine for maintaining homeostasis. When homeostasis is disrupted by infections or tissue injuries, IL-6 is produced immediately and contributes to host defense against such emergent stress through activation of acute-phase and immune responses. However, dysregulated excessive and persistent synthesis of IL-6 has a pathological effect on, respectively, acute systemic inflammatory response syndrome and chronic immune-mediated diseases.

ELISA
- Standard curve
  Example of Human IL-6 standard curve in Assay Diluent FE6.
- Linearity
  The concentrations of Human IL-6 were measured and interpolated from the target standard curves and corrected for sample dilution.
  Sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 6 days (0.1%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 352.1 ng/mL in stimulated human PBMC supernatant.
- Linearity
  The concentrations of Human IL-6 were measured and interpolated from the target standard curves and corrected for sample dilution. Sample is undiluted samples are as follows: human PBMC cells culture for 6 days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 113.7 pg/mL in unstimulated human PBMC supernatant.
- Linearity
  The concentrations of IL-6 were measured and interpolated from the target standard curves and corrected for sample dilution.
  #1 sample is undiluted samples are as follows: monkey PBMC cells stimulated with 10ug/ml PHA for 5days (2%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3573.76 pg/mL in stimulated monkey PBMC supernatant.
  #2 sample is undiluted samples are as follows: monkey PBMC cells culture for 5days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 34.63 pg/mL in unstimulated monkey PBMC supernatant.
- To assess the linearity of the assay, three samples were spiked with high concentrations of Human IL-6 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
- Leading Competitor comparison
- Protocol Diagram