产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
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免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Hu纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
Precision
Intra-assay: 3.6%；
Inter-assay: 7.2%Sample type
Serum，PlasmaAssay type
Sandwich (quantitative)Sensitivity
0.466 pg/mlRange
2.1 pg/mL – 136 pg/mLRecovery
Serum: 109%
Plasma: 88%Assay time
60 minutesSpecies reactivity
HumanNIBSC convert
The dose response curve of the International Standard (21/308) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (21/308) units, use the following equation: NIBSC (21/308) approximate value (IU/mL) = 0.142 x OneStep Human IL-6 value (pg/mL).
Interleukin 6 (IL-6) is a prototypical cytokine for maintaining homeostasis. When homeostasis is disrupted by infections or tissue injuries, IL-6 is produced immediately and contributes to host defense against such emergent stress through activation of acute-phase and immune responses. However, dysregulated excessive and persistent synthesis of IL-6 has a pathological effect on, respectively, acute systemic inflammatory response syndrome and chronic immune-mediated diseases.
ELISA
Standard curve
Example of Human IL-6 standard curve in Assay Diluent #12E6.Linearity
The concentrations of Human IL-6 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 6 days (0.1%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 352.1 ng/mL in stimulated human PBMC supernatant.Linearity
The concentrations of Human IL-6 were measured and interpolated from the target standard curves and corrected for sample dilution. Sample is undiluted samples are as follows: human PBMC cells culture for 6 days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 113.7 pg/mL in unstimulated human PBMC supernatant.To assess the linearity of the assay, two samples were spiked with high concentrations of Human IL-6 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Leading Competitor comparison
Protocol Diagram
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