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抗原名称
IgG免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Rb纯化方式
Protein A储存条件
2 to 8 °C as supplied.
Precision
Intra-assay: 3.7%;
Inter-assay: 8.2%
Sample type
Cell culture supernatant, Serum, PlasmaAssay type
Sandwich (quantitative)Sensitivity
0.036 ng/mlRange
0.156 ng/ml - 10 ng/mlRecovery
Cell culture supernatant 80%
Serum 113%Assay time
60 minutesSpecies reactivity
rabbitCROSS REACTIVITY
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INTERFERENCE
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NIBSC convert
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The rabbit immune system generates antibody diversity and optimizes affinity by mechanisms different from those of mice and other rodents. Compared with traditional mouse mAbs, the rabbit mAbs have advantages in diagnostics with high affinity and specificity toward antigens, more diverse epitope recognition, and greatly improved response to small-size epitopes and mouse antigens. The rabbit immunoglobulin heavy chain (IGH) locus contains over 200 IGH variable germline genes, with over 50% have been found to be “non-functional”. Also, more than 50 IG Kappa V and 17 IG Lambda V functional genes were identified with genomic sequencing (IMGT database). The diversity of rabbit VH repertoire is more limited, and the V light (VL) repertoire is more diverse than that in mice and humans. Most rabbit antibodies are derived from the IGHV1 gene. In addition, the rabbit VL repertoire displays a larger complementarity-determining region (CDR)-L3 loop length than its human and mouse counterparts. Rabbit serum and plasma samples contain around 10 mg/ml of IgG. The use of hybridoma technology or other platforms (e.g., phase display, single B cell antibody technology) for the production of rabbit monoclonal antibodies has created the requirement for a fast and simple procedure for quantifying antibody production.
ELISA
Protocol Diagram
Standard curve
Example of Rabbit IgG standard curve in Assay Diluent #1.
Linearity
#1 The concentrations of Rabbit IgG were measured and interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: Rabbit B cell culture supernatant (0.5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1.41 μg/mL in Rabbit B cell culture supernatant.
#2 sample is undiluted samples are as follows: Rabbit Plasma-Hepairn was 2.5×10^5 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3.9 mg/mL in Rabbit Plasma-Hepairn.
Leading Competitor comparison
Linearity
The concentrations of Rabbit IgG were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Rabbit serum was 2.5×10^5fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 4.5mg/mL in Rabbit serum.
#2 sample is undiluted samples are as follows: Rabbit Plasma-Hepairn was 2.5×10^5fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 5.4 mg/mL in Rabbit Plasma-Hepairn
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