Anti-Rabbit HRP-DAB IHC detection kit

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货号： S0C2031

Datasheet COA

价格： ￥500
规格：
5ml(for50slides)100ml(for1000slides)
数量：

大包装询价

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产品规格

宿主来源

Goat
抗原名称

Rabbit IgG (H+L)
免疫原

Rabbit IgG
抗体类型

Goat pAb
应用

IHC-P
反应种属 ?

Rb
纯化方式

Immunogen Affinity
标记

HRP
性状

Liquid
储存条件

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

稀释度

应用	稀释度
IHC-P	Ready-to-use

背景介绍

Anti-Rabbit HRP-DAB IHC detection kit provides on step detection protocol, short incubation time and faster turnaround. Prior to staining the formalin-fixed paraffin tissue sections should be deparaffinized and hydrated following heat-induced epitope retrieval or enzyme pretreatment. Endogenous peroxidase should be blocked. Then add primary antibody and incubate at optimal titration and condition. After that add HRP Goat anti-Rabbit IgG (H+L) (Ready-to-use, specific for IHC) and incubate for 10-15 minutes. HRP will catalyze DAB to form visible brown deposit at the antigen site. When color development achieved satisfactory level, the slides are washed in water to stop reaction. The stained slides may be mounted with either aqueous mounting or organic mounting medium.

组分和操作说明
- Immunohistochemistry protocol:
  
  Cut and mount sections on slides coated with a suitable tissue adhesive.
  De-paraffinize sections in xylene or xylene substitutes.
  Re-hydrate through graded alcohols.
  Wash slides in distilled H2O.
  Perform antigen retrieval as required.
  Wash slides in distilled H2O.
  Quench endogenous peroxidase using Hydrogen Peroxide Block for 5-10 minutes.
  Wash in wash buffer for 3 x 3 minutes.
  Incubate with optimally diluted primary antibody.
  Wash in wash buffer for 3 x 3 minutes.
  Incubate with HRP Goat anti-Rabbit IgG (H+L) (Ready-to-use, specific for IHC) for 30 minutes.
  Wash in wash buffer for 3 x 3 minutes.
  Add DAB working solution (DAB substrate diluted with DAB dilution buffer) until color development finish.
  Rinse slides in water.
  Add 100μl Haematoxylin and incubate for 3 minutes, then rinse slides in water.
  Clear and mount sections.
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney.
  Anti-Vimentin antibody (S0B2254) was used at 1/500 dilution, followed by a HRP Goat anti-Rabbit IgG (H+L) (Ready-to-use, specific for IHC).
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon.
  Anti-EpCAM antibody (S0B2028) was used at 1/500 dilution, followed by a HRP Goat anti-Rabbit IgG (H+L) (Ready-to-use, specific for IHC).
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.