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产品中心 二抗试剂盒 IHC显色试剂盒

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Anti-Rabbit HRP-DAB IHC detection kit

价格 ￥500.00 （ 1-2周）
货号 S0C2031
规格
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产品介绍

产品介绍 引用文献(2) 评论(0)

产品规格

  • 宿主来源

    Goat

  • 抗原名称

    Rabbit IgG (H+L)

  • 免疫原

    Rabbit IgG

  • 抗体类型

    Goat pAb

  • 反应种属 ?

    Rb

  • 纯化方式

    Immunogen Affinity

  • 标记

    HRP

  • 性状

    Liquid

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

  • 应用

  • 稀释度

    应用 稀释度
    IHC-P Ready-to-use
背景介绍

  • Anti-Rabbit HRP-DAB IHC detection kit provides on step detection protocol, short incubation time and faster turnaround.

  • 组分和操作说明

    • Immunohistochemistry protocol:

      Cut and mount sections on slides coated with a suitable tissue adhesive.

      De-paraffinize sections in xylene or xylene substitutes.

      Re-hydrate through graded alcohols.

      Wash slides in distilled H2O.

      Perform antigen retrieval as required.

      Wash slides in distilled H2O.

      Quench endogenous peroxidase using Hydrogen Peroxide Block for 5-10 minutes.

      Wash in wash buffer for 3 x 3 minutes.

      Incubate with optimally diluted primary antibody.

      Wash in wash buffer for 3 x 3 minutes.

      Incubate with HRP Goat anti-Rabbit IgG (H+L) (Ready-to-use, specific for IHC) for 30 minutes.

      Wash in wash buffer for 3 x 3 minutes.

      Add DAB working solution (DAB substrate diluted with DAB dilution buffer) until color development finish.

      Rinse slides in water.

      Add 100μl Haematoxylin and incubate for 3 minutes, then rinse slides in water.

      Clear and mount sections.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney.
      Anti-Vimentin antibody (S0B2254) was used at 1/500 dilution, followed by a HRP Goat anti-Rabbit IgG (H+L) (Ready-to-use, specific for IHC).
      Counterstained with hematoxylin.
      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon.

      Anti-EpCAM antibody (S0B2028) was used at 1/500 dilution, followed by a HRP Goat anti-Rabbit IgG (H+L) (Ready-to-use, specific for IHC).

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

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