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抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
应用
mIHC
稀释度
应用 稀释度 mIHC 1:200-1:2000
PD-L1, CK-Pan, CD8α, CD4, CD163 Antibody Panel contains 5 recombinant monoclonal antibodies against PD-L1, CK-Pan, CD8α, CD4, CD163. They are provided as a sampler panel.
Panel contains:
- S-RMab® PD-L1 Recombinant Rabbit mAb (SDT-R017) S0B2134
- S-RMab® CK-Pan Recombinant Rabbit mAb (SDT-P001) S0B2118
- CD8α Recombinant Mouse mAb (SDT-126-8) S0B2148
- S-RMab® CD163 Recombinant Rabbit mAb (SDT-R164) S0B2194 - CD4 Recombinant Rabbit mAb (SDT-R163) S0B2179
组分和操作说明
多重免疫组化
mIHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma (A). Anti-PD-L1 Rabbit mAb (S0B2134) was used at 1/200 dilution (White, B), Anti-CD4 Rabbit mAb (S0B2179) was used at 1/200 dilution (Yellow, C), Anti-CD8α Mouse mAb (S0B2148) was used at 1/500 dilution (Orange, D), Anti-CD163 Rabbit mAb (S0B2194) was used at 1/2000 dilution (Green, E), and Anti-CK-Pan Rabbit mAb (S0B2118) was used at 1/1000 dilution (magenta, F), followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with mIHC staining protocol.
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