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抗体类型
Recombinant mAb应用
mIHC反应种属 ?
Hu标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用 | 稀释度 |
---|---|
mIHC | 1:50-1:200 |
CK-Pan, CD45, CD31 Antibody Panel contains 4 recombinant monoclonal antibodies against CK-Pan, CD45, CD31. They are provided as a sampler panel.
Panel contains:
- S-RMab® CK-Pan Recombinant Rabbit mAb (SDT-P001) S0B2118
- S-RMab® CD45 Recombinant Rabbit mAb (SDT-R035) S0B2087
- CD31 Recombinant Rabbit mAb (SDT-008-17) S0B2009
组分和操作说明
多重免疫组化
mIHC shows positive staining in paraffin-embedded human squamous cell carcinoma. Anti- CK-Pan Rabbit mAb (S0B2118) was used at 1/200 dilution (Green), Anti-CD45 Rabbit mAb (S0B2087) was used at 1/40 dilution (Yellow), and Anti-CD31 Rabbit mAb (S0B2009) was used at 1/100 dilution (Red), followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with mIHC staining protocol.
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