Anti-Rabbit and Mouse HRP&DAB IHC detection kit

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货号： S0C1001

Datasheet COA

价格： ￥1,000
规格：
5ml (for 50 slides)50ml (for500 slides)100ml (for1000 slides)
数量：

大包装询价

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产品规格

宿主来源

Goat
克隆号

N/A
抗体类型

pAb
应用

IHC-P
反应种属 ?

Rb, Ms
纯化方式

Protein A
标记

HRP
性状

Liquid
储存条件

12 months from date of receipt, 2 to 8 °C as supplied

稀释度

应用	稀释度
IHC-P	N/A

背景介绍

Anti-Rabbit and Mouse HRP-DAB IHC detection kit utilized the newest biotin-free polymerization technology to prepare super sensitive polymeric peroxidase linked conjugates. It utilizes a novel controlled and compact enzyme polymerization technology to achieve higher sensitivity without background. It provides on step detection protocol, superior sensitivity and specificity, short incubation time and faster turnaround.
Prior to staining the formalin-fixed paraffin tissue sections should be deparaffinized and hydrated following heat-induced epitope retrieval or enzyme pretreatment. Endogenous peroxidase should be blocked. Then add primary antibody and incubate at optimal titration and condition. After that add OneStep polymer HRP Goat anti-Rabbit and Mouse IgG (H+L) (specific for IHC) and incubate for 15-30 minutes. Polymer HRP will catalyze DAB to form visible brown deposit at the antigen site. When color development achieved satisfactory level, the slides are washed in water to stop reaction. The stained slides may be mounted with either aqueous mounting or organic mounting medium.

组分和操作说明
- Immunohistochemistry protocol:
  1. Cut and mount sections on slides coated with a suitable tissue adhesive.
  2. De-paraffinize sections in xylene or xylene substitutes.
  3. Re-hydrate through graded alcohols.
  4. Wash slides in distilled H2O.
  5. Perform antigen retrieval as required.
  6. Wash slides in distilled H2O.
  7. Quench endogenous peroxidase using Hydrogen Peroxide Block for 5-10 minutes.
  8. Wash in wash buffer for 3 x 3 minutes.
  9. Incubate with optimally diluted primary antibody.
  10. Wash in wash buffer for 3 x 3 minutes.
  11. Incubate with ready to use Goat anti-Rabbit&Mouse HRP Conjugate for 15-30 minutes.
  12. Wash in wash buffer for 3 x 3 minutes.
  13. Add DAB working solution (DAB substrate diluted with DAB dilution buffer) until color development finish.
  14. Rinse slides in water.
  15. Add 100ul Haematoxylin and incubate for 3 minutes, then rinse slides in water.
  16. Clear and mount sections
免疫组化
- IHC shows positive staining in paraffin-embedded human stomach. Anti-Claudin18.2 antibody(S0B2069) was used at 1/1000 dilution, followed by anti-Rabbit and Mouse HRP-DAB IHC detection kit. Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Claudin18.2 antibody(S0B2069) was used at 1/1000 dilution, followed by anti-Rabbit and Mouse HRP-DAB IHC detection kit. Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.