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Biotin Mouse Anti-Human CD42a Antibody (S-4391)

Platelet glycoprotein IX,GP-IX,GPIX,Glycoprotein 9,GP9

价格 900.00 供应商现货 : 3-5个工作日
货号 S0B8886
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产品介绍 评论(0)

产品规格
  • 宿主来源

    Mouse
  • 抗原名称

    CD42a
  • 分子别名

    Platelet glycoprotein IX; GP-IX; GPIX; Glycoprotein 9; GP9
  • 细胞定位

    Membrane
  • Accession

    P14770
  • 克隆号

    S-4391
  • 抗体类型

    Mouse mAb
  • 抗体同种型

    IgG1,k
  • 反应种属 ?

    Hu
  • 阳性样本

    Fresh human peripheral blood cells
  • 纯化方式

    Protein G
  • 浓度

    0.2 mg/ml
  • 标记

    Biotin
  • 性状

    Liquid
  • 缓冲体系

    PBS pH7.4, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

  • 应用

    FCM

  • 稀释度

    应用 稀释度 推荐种属
    FCM 5μl per million cells in 100μl volume Hu
背景介绍
  • CD42a, also known as glycoprotein IX (GPIX), is an essential subunit of the platelet surface glycoprotein Ib-IX-V complex. This complex serves as the primary receptor for von Willebrand factor (vWF) and plays a central role in platelet adhesion, thrombus formation, and primary hemostasis. CD42a non-covalently associates with other subunits such as glycoprotein Ibα (GPIbα) and glycoprotein Ibβ (GPIbβ) to maintain the structural stability and proper membrane expression of the receptor complex. Upon vascular injury, the CD42a-containing complex rapidly binds exposed vWF, mediating firm platelet adhesion to the subendothelial matrix under high shear stress and initiating downstream signaling that leads to platelet activation and aggregation. Mutations in the CD42a gene (GP9) cause the rare autosomal recessive disorder Bernard-Soulier syndrome, characterized by giant platelets, thrombocytopenia, and a severe bleeding tendency. Furthermore, as a platelet-specific marker, CD42a holds significant value in flow cytometry, quality assessment of platelet transfusion, and research into the pathological mechanisms of thrombotic disorders.

  • 流式分析

    • Flow cytometric analysis of human CD42a expression on fresh human peripheral blood cells. Fresh human peripheral blood cells were stained with Brilliant Violet 421™ Mouse Anti-Human CD41 Antibody and either Biotin IgG1, k Isotype Control (left panel) or SDT Biotin Mouse Anti-Human CD42a Antibody (right panel) at 5 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

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