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Mouse抗原名称
H-2Kd分子别名
H-2 class I histocompatibility antigen, K-D alpha chain; H-2K(D); H2-K1; H2-K细胞定位
MembraneAccession
P01902克隆号
SF1-1.1抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
BALB/c mouse splenocytes纯化方式
Protein A浓度
0.5 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
H-2Kd protein is a major histocompatibility complex (MHC) class I molecule expressed in mice, particularly in BALB/c strains. It plays a critical role in antigen presentation by binding to antigenic peptides derived from pathogens, such as influenza nucleoprotein, and presenting them to CD8+ T cells to initiate an immune response. The crystal structure of H-2Kd in complex with beta-2 microglobulin and an influenza-derived peptide has been elucidated, revealing specific binding motifs and the structural basis for peptide recognition. This understanding is crucial for studies on immune responses and vaccine development.
流式分析
Flow cytometric analysis of mouse H-2Kd expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Biotin Mouse IgG2a, κ Isotype Control (black line histogram) or SDT Biotin Mouse Anti-Mouse H-2Kd Antibody (red line histogram) at 2 μl/test followed by Sav-iFluor 488, cells without incubation with primary antibody and secondary antibody (blue line histogram) was used as unlabeled control. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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