热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 流式试剂&相关 死活染料

所有图片（1/2）

Fixable Viability Dye 448

价格 ￥630.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B88807
规格
数量
收藏 分享
产品介绍

产品介绍 评论(0)

产品规格

  • 储存条件

    12 months from date of receipt, -20 °C as supplied

背景介绍

  • Fixable Viability Dye 448 is excited by the UV laser (with an excitation maximum of 350 nm) and has fluorescence emission maximum at 448 nm, Fixable Viability Dye 448 has similar emission to DAPI.

    Fixable Viability Dye 448 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications.

    Each lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.

操作步骤

  • 1.Wash cells one time in PBS buffer.

    2.Add 1 µl of Fixable Viability Dye 448 Stock Solution for each 0.5~1 ml of cell suspension (1:500~1:1000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.

    3.Resuspend cells at 1*10^7 cells/ml in Fixable Viability Dye 448, Incubate the mixture for 10-15 minutes at room temperature protected from light.

    4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.

    5.Resuspend the cells in Stain Buffer.

    Note: This protocol applicable to Human PBMC (human peripheral blood mononuclear cells) Dying, the reagent be titrated for optimal performance for different cell types.

  • 组分和操作说明

    • For reconstitution, pre-warm the kit to room temperature; add 100 µl of DMSO to one vial of Fixable Viability Dye 448 and mix until fully dissolved. After reconstitution, store aliquots at -20°C. Repeated freezing and thawing are not recommended. Use up within 3 months. Repeated freezing and thawing are not recommended.

      Note: We recommend dissolving it in 100 µL of DMSO. The DMSO was overfilled to ensure complete dissolution of the required amount.

  • 流式分析

    • Human PBMCs (human peripheral blood mononuclear cells) were stained with Fixable Viability Dye 448 and analyzed before fixation (middle Panels) or after fixation (right Panels). Cells alone, without Fixable Viability Dye 448 staining (left Panels). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

评论(0)