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产品中心 流式试剂&相关 死活染料

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Fixable Viability Dye 515

价格 ￥630.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B88804
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产品介绍

产品介绍 评论(0)

产品规格

  • 储存条件

    12 months from date of receipt, -20 °C as supplied.

背景介绍

  • Fixable Viability Dye 515 is excited by the Blue laser (with an excitation maximum of 488 nm) and has fluorescence emission maximum at 515 nm, Fixable Viability Dye 515 has similar emission to FITC.

    Fixable Viability Dye 515 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications.

    Each lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.

操作步骤

  • 1.Wash cells one time in PBS buffer.

    2.Add 1 µl of Fixable Viability Dye 515 Stock Solution for each 1~2 ml of cell suspension (1:1000~1:2000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.

    3.Resuspend cells at 1*10^7 cells/ml in Fixable Viability Dye 515, Incubate the mixture for 10-15 minutes at room temperature protected from light.

    4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.

    5.Resuspend the cells in Stain Buffer.

    Note: This protocol applicable to mouse splenocytes Dying, the reagent be titrated for optimal performance for different cell types. Stain, fix and permeabilize cells as desired for downstream applications.

  • 组分和操作说明

    • For reconstitution, pre-warm the kit to room temperature; add 100 µl of DMSO to one vial of Fixable Viability Dye 515 and mix until fully dissolved. After reconstitution, store aliquots at -20°C. Repeated freezing and thawing are not recommended. Use up within 3 months. Repeated freezing and thawing are not recommended.

      Note: We recommend dissolving it in 100 µL of DMSO. The DMSO was overfilled to ensure complete dissolution of the required amount.

  • 流式分析

    • Mouse splenocytes were stained with Fixable Viability Dye 515 and analyzed before fixation (middle Panels) or after fixation (right Panels). Cells alone, without Fixable Viability Dye 515 staining (left Panels). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

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