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产品中心 流式试剂&相关 死活染料

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Fixable Viability Dye 452

价格 ￥630.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B88801
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产品介绍

产品介绍 评论(0)

产品规格

  • 储存条件

    12 months from date of receipt, -20 °C as supplied

背景介绍

  • Fixable Viability Dye 452 is excited by the Violet laser (with an excitation maximum of 405 nm) and has fluorescence emission maximum at 452 nm, Fixable Viability Dye 452 has similar emission to Brilliant Violet 421™.

    Fixable Viability Dye 452 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, Each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications.

    Each lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.

操作步骤

  • 1.Wash cells one time in PBS buffer.

    2.Add 1 µl of Fixable Viability Dye 452 Stock Solution for each 0.5~1 ml of cell suspension/PBS (1:500~1:1000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.

    3.Resuspend cells at 1*10^7 cells/ml in Fixable Viability Dye 452, Incubate the mixture for 10-15 minutes at room temperature protected from light.

    4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.

    5.Resuspend the cells in Stain Buffer.

    Note: This protocol applicable to Human PBMCs (human peripheral blood mononuclear cells) Dying, the reagent be titrated for optimal performance for different cell types. Stain, fix and permeabilize cells as desired for downstream applications.

  • 组分和操作说明

    • For reconstitution, pre-warm the kit to room temperature; add 100 µl of DMSO to one vial of Fixable Viability Dye 452 and mix until fully dissolved. After reconstitution with DMSO, store the stock solution at -20°C in small aliquots. Use up within 3 months. Repeated freezing and thawing are not recommended.

      Note: We recommend dissolving it in 100 µL of DMSO. The DMSO was overfilled to ensure complete dissolution of the required amount.

  • 流式分析

    • Human PBMCs (human peripheral blood mononuclear cells) were stained with Fixable Viability Dye 452 and analyzed before fixation (Middle Panels) or after fixation (Right Panels). Cells alone, without Fixable Viability Dye 452 staining (Left Panels). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

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